Arginine and nitric oxide metabolism in critically ill septic pediatric patients

Zvi Argaman, Vernon R. Young, Natan Noviski, Luis Castillo-Rosas, Xiao Ming Lu, David Zurakowski, Mehrengise Cooper, Caroline Davison, John F. Tharakan, Alfred Ajami, Leticia Castillo

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Objective: To investigate whole body, in vivo arginine metabolism and nitric oxide synthesis rates in septic, critically ill pediatric patients. Design: Prospective study. Setting: Pediatric intensive care unit at a general hospital. Patients: Ten consecutive septic patients age 6-16 yrs. Interventions: Septic patients received an 8-hr primed, constant intravenous tracer infusion of L-[guanidino-15N2]arginine, L-[1-13C]leucine, and [13C]urea. A 24-hr urine collection was obtained for determination of [15N]nitrate enrichment (15NO3-) and urinary nitrogen. The next day they received an infusion of L-[5-13C]arginine and L-[5-13C-ureido, 5,5,2H2]citrulline. Blood samples were obtained for determination of plasma isotopic enrichment of the tracers given and of derived [15N]citrulline (nitric oxide synthesis), L-[13C-guanidino 5,5, 2H2]arginine (M+3 arg) (arginine synthesis), and [15N2]urea (urea formation). Data are compared with historic controls from studies in healthy young adults. Measurements and Main Results: Plasma arginine fluxes were 67 ± 21 and 72 ±17 μmol·kg-1·hr-1 respectively, for the [15N2 guanidino] and the [13C] arginine labels, which were not different from reported adult values. The rates of arginine oxidation were 22.9 ± 10.8 μmol·kg-1·hr-1 and were higher than de novo arginine synthesis rates of 9.6 ± 4.2 μmol·kg-1·hr-1 (p < .01); therefore, these patients were in a negative arginine balance. The rates of nitric oxide synthesis as estimated by the [15N]citrulline method were 1.58 ± 0.69 μmol·kg-1·hr-1 for septic patients and higher (p < .05) than values of 0.96 ± 0.1 μmol·kg-1·hr-1 in healthy adults. Septic patients were in a negative protein (leucine) balance of about -1.00 ± 0.40 g·kg-1·day-1. Conclusions: Homeostasis of plasma arginine in septic patients was impaired compared with reported adult values. The rates of arginine oxidation were increased whereas de novo net arginine synthesis was unchanged, leading to a negative arginine balance. The rates of nitric oxide synthesis and the fraction of plasma arginine used for nitric oxide and urea formation were increased. These findings suggest that under condition of sepsis, arginine becomes essential in critically ill children.

Original languageEnglish (US)
Pages (from-to)591-597
Number of pages7
JournalCritical Care Medicine
Volume31
Issue number2
DOIs
StatePublished - Feb 1 2003
Externally publishedYes

Fingerprint

Critical Illness
Arginine
Nitric Oxide
Pediatrics
Citrulline
Urea
Leucine
Urine Specimen Collection
Pediatric Intensive Care Units
Intravenous Infusions
General Hospitals
Nitrates
Young Adult
Sepsis
Homeostasis
Nitrogen

Keywords

  • Amino acid
  • Arginine
  • Children
  • Citrulline
  • Kinetics
  • Leucine
  • Metabolism
  • Nitrate
  • Nitric oxide
  • Oxidation
  • Pediatric sepsis
  • Stable isotopes
  • Synthesis rate

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Argaman, Z., Young, V. R., Noviski, N., Castillo-Rosas, L., Lu, X. M., Zurakowski, D., ... Castillo, L. (2003). Arginine and nitric oxide metabolism in critically ill septic pediatric patients. Critical Care Medicine, 31(2), 591-597. https://doi.org/10.1097/01.CCM.0000050291.37714.74

Arginine and nitric oxide metabolism in critically ill septic pediatric patients. / Argaman, Zvi; Young, Vernon R.; Noviski, Natan; Castillo-Rosas, Luis; Lu, Xiao Ming; Zurakowski, David; Cooper, Mehrengise; Davison, Caroline; Tharakan, John F.; Ajami, Alfred; Castillo, Leticia.

In: Critical Care Medicine, Vol. 31, No. 2, 01.02.2003, p. 591-597.

Research output: Contribution to journalArticle

Argaman, Z, Young, VR, Noviski, N, Castillo-Rosas, L, Lu, XM, Zurakowski, D, Cooper, M, Davison, C, Tharakan, JF, Ajami, A & Castillo, L 2003, 'Arginine and nitric oxide metabolism in critically ill septic pediatric patients', Critical Care Medicine, vol. 31, no. 2, pp. 591-597. https://doi.org/10.1097/01.CCM.0000050291.37714.74
Argaman Z, Young VR, Noviski N, Castillo-Rosas L, Lu XM, Zurakowski D et al. Arginine and nitric oxide metabolism in critically ill septic pediatric patients. Critical Care Medicine. 2003 Feb 1;31(2):591-597. https://doi.org/10.1097/01.CCM.0000050291.37714.74
Argaman, Zvi ; Young, Vernon R. ; Noviski, Natan ; Castillo-Rosas, Luis ; Lu, Xiao Ming ; Zurakowski, David ; Cooper, Mehrengise ; Davison, Caroline ; Tharakan, John F. ; Ajami, Alfred ; Castillo, Leticia. / Arginine and nitric oxide metabolism in critically ill septic pediatric patients. In: Critical Care Medicine. 2003 ; Vol. 31, No. 2. pp. 591-597.
@article{c61bcaac106b4c3fbc0cb12b1e317859,
title = "Arginine and nitric oxide metabolism in critically ill septic pediatric patients",
abstract = "Objective: To investigate whole body, in vivo arginine metabolism and nitric oxide synthesis rates in septic, critically ill pediatric patients. Design: Prospective study. Setting: Pediatric intensive care unit at a general hospital. Patients: Ten consecutive septic patients age 6-16 yrs. Interventions: Septic patients received an 8-hr primed, constant intravenous tracer infusion of L-[guanidino-15N2]arginine, L-[1-13C]leucine, and [13C]urea. A 24-hr urine collection was obtained for determination of [15N]nitrate enrichment (15NO3-) and urinary nitrogen. The next day they received an infusion of L-[5-13C]arginine and L-[5-13C-ureido, 5,5,2H2]citrulline. Blood samples were obtained for determination of plasma isotopic enrichment of the tracers given and of derived [15N]citrulline (nitric oxide synthesis), L-[13C-guanidino 5,5, 2H2]arginine (M+3 arg) (arginine synthesis), and [15N2]urea (urea formation). Data are compared with historic controls from studies in healthy young adults. Measurements and Main Results: Plasma arginine fluxes were 67 ± 21 and 72 ±17 μmol·kg-1·hr-1 respectively, for the [15N2 guanidino] and the [13C] arginine labels, which were not different from reported adult values. The rates of arginine oxidation were 22.9 ± 10.8 μmol·kg-1·hr-1 and were higher than de novo arginine synthesis rates of 9.6 ± 4.2 μmol·kg-1·hr-1 (p < .01); therefore, these patients were in a negative arginine balance. The rates of nitric oxide synthesis as estimated by the [15N]citrulline method were 1.58 ± 0.69 μmol·kg-1·hr-1 for septic patients and higher (p < .05) than values of 0.96 ± 0.1 μmol·kg-1·hr-1 in healthy adults. Septic patients were in a negative protein (leucine) balance of about -1.00 ± 0.40 g·kg-1·day-1. Conclusions: Homeostasis of plasma arginine in septic patients was impaired compared with reported adult values. The rates of arginine oxidation were increased whereas de novo net arginine synthesis was unchanged, leading to a negative arginine balance. The rates of nitric oxide synthesis and the fraction of plasma arginine used for nitric oxide and urea formation were increased. These findings suggest that under condition of sepsis, arginine becomes essential in critically ill children.",
keywords = "Amino acid, Arginine, Children, Citrulline, Kinetics, Leucine, Metabolism, Nitrate, Nitric oxide, Oxidation, Pediatric sepsis, Stable isotopes, Synthesis rate",
author = "Zvi Argaman and Young, {Vernon R.} and Natan Noviski and Luis Castillo-Rosas and Lu, {Xiao Ming} and David Zurakowski and Mehrengise Cooper and Caroline Davison and Tharakan, {John F.} and Alfred Ajami and Leticia Castillo",
year = "2003",
month = "2",
day = "1",
doi = "10.1097/01.CCM.0000050291.37714.74",
language = "English (US)",
volume = "31",
pages = "591--597",
journal = "Critical Care Medicine",
issn = "0090-3493",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Arginine and nitric oxide metabolism in critically ill septic pediatric patients

AU - Argaman, Zvi

AU - Young, Vernon R.

AU - Noviski, Natan

AU - Castillo-Rosas, Luis

AU - Lu, Xiao Ming

AU - Zurakowski, David

AU - Cooper, Mehrengise

AU - Davison, Caroline

AU - Tharakan, John F.

AU - Ajami, Alfred

AU - Castillo, Leticia

PY - 2003/2/1

Y1 - 2003/2/1

N2 - Objective: To investigate whole body, in vivo arginine metabolism and nitric oxide synthesis rates in septic, critically ill pediatric patients. Design: Prospective study. Setting: Pediatric intensive care unit at a general hospital. Patients: Ten consecutive septic patients age 6-16 yrs. Interventions: Septic patients received an 8-hr primed, constant intravenous tracer infusion of L-[guanidino-15N2]arginine, L-[1-13C]leucine, and [13C]urea. A 24-hr urine collection was obtained for determination of [15N]nitrate enrichment (15NO3-) and urinary nitrogen. The next day they received an infusion of L-[5-13C]arginine and L-[5-13C-ureido, 5,5,2H2]citrulline. Blood samples were obtained for determination of plasma isotopic enrichment of the tracers given and of derived [15N]citrulline (nitric oxide synthesis), L-[13C-guanidino 5,5, 2H2]arginine (M+3 arg) (arginine synthesis), and [15N2]urea (urea formation). Data are compared with historic controls from studies in healthy young adults. Measurements and Main Results: Plasma arginine fluxes were 67 ± 21 and 72 ±17 μmol·kg-1·hr-1 respectively, for the [15N2 guanidino] and the [13C] arginine labels, which were not different from reported adult values. The rates of arginine oxidation were 22.9 ± 10.8 μmol·kg-1·hr-1 and were higher than de novo arginine synthesis rates of 9.6 ± 4.2 μmol·kg-1·hr-1 (p < .01); therefore, these patients were in a negative arginine balance. The rates of nitric oxide synthesis as estimated by the [15N]citrulline method were 1.58 ± 0.69 μmol·kg-1·hr-1 for septic patients and higher (p < .05) than values of 0.96 ± 0.1 μmol·kg-1·hr-1 in healthy adults. Septic patients were in a negative protein (leucine) balance of about -1.00 ± 0.40 g·kg-1·day-1. Conclusions: Homeostasis of plasma arginine in septic patients was impaired compared with reported adult values. The rates of arginine oxidation were increased whereas de novo net arginine synthesis was unchanged, leading to a negative arginine balance. The rates of nitric oxide synthesis and the fraction of plasma arginine used for nitric oxide and urea formation were increased. These findings suggest that under condition of sepsis, arginine becomes essential in critically ill children.

AB - Objective: To investigate whole body, in vivo arginine metabolism and nitric oxide synthesis rates in septic, critically ill pediatric patients. Design: Prospective study. Setting: Pediatric intensive care unit at a general hospital. Patients: Ten consecutive septic patients age 6-16 yrs. Interventions: Septic patients received an 8-hr primed, constant intravenous tracer infusion of L-[guanidino-15N2]arginine, L-[1-13C]leucine, and [13C]urea. A 24-hr urine collection was obtained for determination of [15N]nitrate enrichment (15NO3-) and urinary nitrogen. The next day they received an infusion of L-[5-13C]arginine and L-[5-13C-ureido, 5,5,2H2]citrulline. Blood samples were obtained for determination of plasma isotopic enrichment of the tracers given and of derived [15N]citrulline (nitric oxide synthesis), L-[13C-guanidino 5,5, 2H2]arginine (M+3 arg) (arginine synthesis), and [15N2]urea (urea formation). Data are compared with historic controls from studies in healthy young adults. Measurements and Main Results: Plasma arginine fluxes were 67 ± 21 and 72 ±17 μmol·kg-1·hr-1 respectively, for the [15N2 guanidino] and the [13C] arginine labels, which were not different from reported adult values. The rates of arginine oxidation were 22.9 ± 10.8 μmol·kg-1·hr-1 and were higher than de novo arginine synthesis rates of 9.6 ± 4.2 μmol·kg-1·hr-1 (p < .01); therefore, these patients were in a negative arginine balance. The rates of nitric oxide synthesis as estimated by the [15N]citrulline method were 1.58 ± 0.69 μmol·kg-1·hr-1 for septic patients and higher (p < .05) than values of 0.96 ± 0.1 μmol·kg-1·hr-1 in healthy adults. Septic patients were in a negative protein (leucine) balance of about -1.00 ± 0.40 g·kg-1·day-1. Conclusions: Homeostasis of plasma arginine in septic patients was impaired compared with reported adult values. The rates of arginine oxidation were increased whereas de novo net arginine synthesis was unchanged, leading to a negative arginine balance. The rates of nitric oxide synthesis and the fraction of plasma arginine used for nitric oxide and urea formation were increased. These findings suggest that under condition of sepsis, arginine becomes essential in critically ill children.

KW - Amino acid

KW - Arginine

KW - Children

KW - Citrulline

KW - Kinetics

KW - Leucine

KW - Metabolism

KW - Nitrate

KW - Nitric oxide

KW - Oxidation

KW - Pediatric sepsis

KW - Stable isotopes

KW - Synthesis rate

UR - http://www.scopus.com/inward/record.url?scp=0037326678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037326678&partnerID=8YFLogxK

U2 - 10.1097/01.CCM.0000050291.37714.74

DO - 10.1097/01.CCM.0000050291.37714.74

M3 - Article

C2 - 12576971

AN - SCOPUS:0037326678

VL - 31

SP - 591

EP - 597

JO - Critical Care Medicine

JF - Critical Care Medicine

SN - 0090-3493

IS - 2

ER -