Arginine and ornithine kinetics in severely burned patients: Increased rate of arginine disposal

Yong Ming Yu, Colleen M. Ryan, Leticia Castillo, Xiao Ming Lu, Louis Beaumier, Ronald G. Tompkins, Vernon R. Young

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Arginine serves multiple roles in the pathophysiological response to burn injury. Our previous studies in burn patients demonstrated a limited net rate of arginine de novo synthesis despite a significantly increased arginine turnover (flux), suggesting that this amino acid is a conditionally indispensable amino acid after major burns. This study used [15N2-guanidino-5,5-2H2]arginine and [5-13C]ornithine as tracers to assess the rate of arginine disposal via its conversion to and subsequent oxidation of ornithine; [5,52H2]proline and [5,5,5-2H3]leucine were also used to assess proline and protein kinetics. Nine severely burned patients were studied during a protein-free fast ("basal" or fast) and total parenteral nutrition (TPN) feedings. Compared with values from healthy volunteers, burn injury significantly increased 1) fluxes of arginine, ornithine, leucine, and proline; 2) arginine-to-ornithine conversion; 3) ornithine oxidation; and 4) arginine oxidation. TPN increased arginine-to-ornithine conversion and proportionally increased irreversible arginine oxidation. The elevated arginine oxidation, with limited net de novo synthesis from its immediate precursors, further implies that arginine is a conditionally indispensable amino acid in severely burned patients receiving TPN.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume280
Issue number3 43-3
StatePublished - Mar 1 2001
Externally publishedYes

Fingerprint

Ornithine
Arginine
Total Parenteral Nutrition
Proline
Amino Acids
Leucine
Parenteral Nutrition
Wounds and Injuries
Burns
Healthy Volunteers
Proteins

Keywords

  • Basal phase
  • De novo synthesis
  • Leucine
  • Oxidation
  • Total parental nutrition

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Arginine and ornithine kinetics in severely burned patients : Increased rate of arginine disposal. / Yu, Yong Ming; Ryan, Colleen M.; Castillo, Leticia; Lu, Xiao Ming; Beaumier, Louis; Tompkins, Ronald G.; Young, Vernon R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 280, No. 3 43-3, 01.03.2001.

Research output: Contribution to journalArticle

Yu, Yong Ming ; Ryan, Colleen M. ; Castillo, Leticia ; Lu, Xiao Ming ; Beaumier, Louis ; Tompkins, Ronald G. ; Young, Vernon R. / Arginine and ornithine kinetics in severely burned patients : Increased rate of arginine disposal. In: American Journal of Physiology - Endocrinology and Metabolism. 2001 ; Vol. 280, No. 3 43-3.
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