Arginine and ornithine kinetics in severely burned patients

Increased rate of arginine disposal

Yong Ming Yu, Colleen M. Ryan, Leticia Castillo, Xiao Ming Lu, Louis Beaumier, Ronald G. Tompkins, Vernon R. Young

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Arginine serves multiple roles in the pathophysiological response to burn injury. Our previous studies in burn patients demonstrated a limited net rate of arginine de novo synthesis despite a significantly increased arginine turnover (flux), suggesting that this amino acid is a conditionally indispensable amino acid after major burns. This study used [15N2-guanidino-5,5-2H2]arginine and [5-13C]ornithine as tracers to assess the rate of arginine disposal via its conversion to and subsequent oxidation of ornithine; [5,52H2]proline and [5,5,5-2H3]leucine were also used to assess proline and protein kinetics. Nine severely burned patients were studied during a protein-free fast ("basal" or fast) and total parenteral nutrition (TPN) feedings. Compared with values from healthy volunteers, burn injury significantly increased 1) fluxes of arginine, ornithine, leucine, and proline; 2) arginine-to-ornithine conversion; 3) ornithine oxidation; and 4) arginine oxidation. TPN increased arginine-to-ornithine conversion and proportionally increased irreversible arginine oxidation. The elevated arginine oxidation, with limited net de novo synthesis from its immediate precursors, further implies that arginine is a conditionally indispensable amino acid in severely burned patients receiving TPN.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume280
Issue number3 43-3
StatePublished - Mar 1 2001
Externally publishedYes

Fingerprint

Ornithine
Arginine
Total Parenteral Nutrition
Proline
Amino Acids
Leucine
Parenteral Nutrition
Wounds and Injuries
Burns
Healthy Volunteers
Proteins

Keywords

  • Basal phase
  • De novo synthesis
  • Leucine
  • Oxidation
  • Total parental nutrition

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Arginine and ornithine kinetics in severely burned patients : Increased rate of arginine disposal. / Yu, Yong Ming; Ryan, Colleen M.; Castillo, Leticia; Lu, Xiao Ming; Beaumier, Louis; Tompkins, Ronald G.; Young, Vernon R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 280, No. 3 43-3, 01.03.2001.

Research output: Contribution to journalArticle

Yu, Yong Ming ; Ryan, Colleen M. ; Castillo, Leticia ; Lu, Xiao Ming ; Beaumier, Louis ; Tompkins, Ronald G. ; Young, Vernon R. / Arginine and ornithine kinetics in severely burned patients : Increased rate of arginine disposal. In: American Journal of Physiology - Endocrinology and Metabolism. 2001 ; Vol. 280, No. 3 43-3.
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