Arginine, citrulline, and nitric oxide metabolism in end-stage renal disease patients

Titus Lau, William Owen, Yong Ming Yu, Natan Noviski, Jeremy Lyons, David Zurakowski, Rita Tsay, Alfred Ajami, Vernon R. Young, Leticia Castillo

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Abstract

The kidneys are thought to be a major site of net de novo arginine synthesis, but the quantitative status of arginine metabolism and its substrate precursor relationship to nitric oxide (NO) synthesis in end stage renal disease (ESRD) patients have not been characterized. We have investigated kinetic aspects of whole body arginine metabolism in six patients with ESRD. They received two pre- and two post-hemodialysis intravenous tracer infusion studies with L-[guanidino-15N2]arginine and L- [13C]leucine during the first study, and L-[5-13C] arginine and L-[5- 13C-ureido,5,5,2H2] citrulline during the second study. Arginine homeostasis in ESRD patients was found to be associated with a lower rate of arginine oxidation, and despite the decrease in renal function, the rate of de novo arginine synthesis appeared to be preserved. Plasma citrulline concentrations and flux were also elevated in these subjects compared with healthy adults. The rate of whole body NO synthesis was increased in the ESRD patients, but apparently not different pre- and post-hemodialysis therapy. The anatomic site(s) responsible for the maintenance of net de novo arginine synthesis and for the elevated NO synthesis and its pathophysiological importance in ESRD remain to be established.

Original languageEnglish (US)
Pages (from-to)1217-1225
Number of pages9
JournalJournal of Clinical Investigation
Volume105
Issue number9
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

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Citrulline
Chronic Kidney Failure
Arginine
Nitric Oxide
Renal Dialysis
Kidney
Intravenous Infusions
Leucine
Homeostasis
Maintenance

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Arginine, citrulline, and nitric oxide metabolism in end-stage renal disease patients. / Lau, Titus; Owen, William; Yu, Yong Ming; Noviski, Natan; Lyons, Jeremy; Zurakowski, David; Tsay, Rita; Ajami, Alfred; Young, Vernon R.; Castillo, Leticia.

In: Journal of Clinical Investigation, Vol. 105, No. 9, 01.01.2000, p. 1217-1225.

Research output: Contribution to journalArticle

Lau, T, Owen, W, Yu, YM, Noviski, N, Lyons, J, Zurakowski, D, Tsay, R, Ajami, A, Young, VR & Castillo, L 2000, 'Arginine, citrulline, and nitric oxide metabolism in end-stage renal disease patients', Journal of Clinical Investigation, vol. 105, no. 9, pp. 1217-1225. https://doi.org/10.1172/JCI7199
Lau, Titus ; Owen, William ; Yu, Yong Ming ; Noviski, Natan ; Lyons, Jeremy ; Zurakowski, David ; Tsay, Rita ; Ajami, Alfred ; Young, Vernon R. ; Castillo, Leticia. / Arginine, citrulline, and nitric oxide metabolism in end-stage renal disease patients. In: Journal of Clinical Investigation. 2000 ; Vol. 105, No. 9. pp. 1217-1225.
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