@article{84bd29588c574866b77aeb8877329e9c,
title = "Arrangement of the Polymerase Complexes inside a Nine-Segmented dsRNA Virus",
abstract = "The arrangement of polymerases inside an unusual nine-segmented dsRNA virus was determined by Kaelber et al. While cryo-EM is typically used to average copies of a protein into one structure, they also compared raw images with synthetic maps to test whether subsets of virions had different arrangements.",
keywords = "RNA-dependent RNA polymerase, Reoviridae, asymmetric reconstruction, cryo-EM reconstruction, single particle",
author = "Kaelber, {Jason T.} and Wen Jiang and Weaver, {Scott C.} and Albert Auguste and Wah Chiu",
note = "Funding Information: We thank Ms. Joanita Jakana for technical support. We thank Drs. Steve Ludtke, Matt Baker, and Michael Schmid for helpful discussions. We further thank Drs. Matt Baker and Qinfen Zhang for sharing pre-publication results. This research was supported by the National Institutes of Health ( P41GM103832 to W.C., K22AI125474 to A.J.A., and R24AI120942 to S.C.W.), and the Welch Foundation ( Q1242 ). A.J.A. is also supported by a Junior faculty award from the Institute for Critical Technology and Applied Science at Virginia Tech and by the USDA National Institute of Food and Agriculture , Hatch VA-160103, project (1020026). We gratefully acknowledge the assistance and computing resources provided by the CIBR Center for Computational and Integrative Biomedical Research of Baylor College of Medicine in the completion of this work. Funding Information: We thank Ms. Joanita Jakana for technical support. We thank Drs. Steve Ludtke, Matt Baker, and Michael Schmid for helpful discussions. We further thank Drs. Matt Baker and Qinfen Zhang for sharing pre-publication results. This research was supported by the National Institutes of Health (P41GM103832 to W.C. K22AI125474 to A.J.A. and R24AI120942 to S.C.W.), and the Welch Foundation (Q1242). A.J.A. is also supported by a Junior faculty award from the Institute for Critical Technology and Applied Science at Virginia Tech and by the USDA National Institute of Food and Agriculture, Hatch VA-160103, project (1020026). We gratefully acknowledge the assistance and computing resources provided by the CIBR Center for Computational and Integrative Biomedical Research of Baylor College of Medicine in the completion of this work. Conceptualization, J.T.K. and A.J.A.; Methodology, J.T.K.; Investigation, J.T.K. W.J. and A.J.A.; Expert Advice, W.J.; Writing, J.T.K. A.J.A. and W.C.; Reviewing, J.T.K. S.C.W. A.J.A. and W.C.; Funding Acquisition, W.C. S.C.W. and A.J.A.; Supervision, S.C.W. and W.C. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",
year = "2020",
month = jun,
day = "2",
doi = "10.1016/j.str.2020.01.011",
language = "English (US)",
volume = "28",
pages = "604--612.e3",
journal = "Structure",
issn = "0969-2126",
publisher = "Cell Press",
number = "6",
}