Artonin E mediates MCL1 down-regulation and sensitizes lung cancer cells to anoikis

Ekkarat Wongpankam, Preedakorn Chunhacha, Varisa Pongrakhananon, Boonchoo Sritularak, Pithi Chanvorachote

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Anoikis, or detachment-induced apoptosis, is recognized as a key inhibitory process of cancer metastasis. Since lung cancer cells possess an ability to resist anoikis, resulting in a high rate of metastasis and death, the present study aimed to investigate the possible anoikis-sensitizing effect of artonin E (AE). Materials and Methods: AE was extracted from bark of Artocarpus gomezianus. Anoikis sensitization of AE was investigated in H460, A549 and H292 human lung cancer cells. The level of anoikis-related proteins was determined by western blot analysis and viable cells were measured by the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) method. Results: AE was shown to enhance anoikis of H460 cells in a dose-dependent manner. We investigated the underlying mechanisms of AE on anoikis sensitization and found that AE sensitized the cells by down-regulating the anti-apoptotic myeloid leukemia cell sequence-1 (MCL1) protein but had no significant effect on other proteins of the B-cell lymphoma-2 (BCL2) family, including BCL2 and BCL2-associated X protein (BAX). Anoikis sensitization of AE was consistently observed in A549 and H292 lung cancer cells. Conclusion: The present study demonstrates a novel activity of AE on lung cancer cell anoikis for the first time which might lead to the development of a new strategy for lung cancer therapy.

Original languageEnglish (US)
Pages (from-to)5343-5351
Number of pages9
JournalAnticancer Research
Volume32
Issue number12
StatePublished - Dec 2012
Externally publishedYes

Fingerprint

Myeloid Cell Leukemia Sequence 1 Protein
Anoikis
Lung Neoplasms
Down-Regulation
B-Cell Lymphoma
Artocarpus
Neoplasm Metastasis
artonin E

Keywords

  • A549
  • Anoikis
  • Artonin E
  • H292
  • H460
  • Hk2 cells
  • Lung cancer cells
  • MCL1
  • Metastasis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wongpankam, E., Chunhacha, P., Pongrakhananon, V., Sritularak, B., & Chanvorachote, P. (2012). Artonin E mediates MCL1 down-regulation and sensitizes lung cancer cells to anoikis. Anticancer Research, 32(12), 5343-5351.

Artonin E mediates MCL1 down-regulation and sensitizes lung cancer cells to anoikis. / Wongpankam, Ekkarat; Chunhacha, Preedakorn; Pongrakhananon, Varisa; Sritularak, Boonchoo; Chanvorachote, Pithi.

In: Anticancer Research, Vol. 32, No. 12, 12.2012, p. 5343-5351.

Research output: Contribution to journalArticle

Wongpankam, E, Chunhacha, P, Pongrakhananon, V, Sritularak, B & Chanvorachote, P 2012, 'Artonin E mediates MCL1 down-regulation and sensitizes lung cancer cells to anoikis', Anticancer Research, vol. 32, no. 12, pp. 5343-5351.
Wongpankam E, Chunhacha P, Pongrakhananon V, Sritularak B, Chanvorachote P. Artonin E mediates MCL1 down-regulation and sensitizes lung cancer cells to anoikis. Anticancer Research. 2012 Dec;32(12):5343-5351.
Wongpankam, Ekkarat ; Chunhacha, Preedakorn ; Pongrakhananon, Varisa ; Sritularak, Boonchoo ; Chanvorachote, Pithi. / Artonin E mediates MCL1 down-regulation and sensitizes lung cancer cells to anoikis. In: Anticancer Research. 2012 ; Vol. 32, No. 12. pp. 5343-5351.
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AU - Sritularak, Boonchoo

AU - Chanvorachote, Pithi

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