Aspalatone Prevents VEGF-Induced Lipid Peroxidation, Migration, Tube Formation, and Dysfunction of Human Aortic Endothelial Cells

Himangshu Sonowal, Pabitra B. Pal, Kirtikar Shukla, Kota Ramana

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Although aspalatone (acetylsalicylic acid maltol ester) is recognized as an antithrombotic agent with antioxidative and antiplatelet potential; its efficacy in preventing endothelial dysfunction is not known. In this study, we examined the antiangiogenic, antioxidative, and anti-inflammatory effect of aspalatone in human aortic endothelial cells (HAECs). Specifically, the effect of aspalatone on VEGF-induced HAECs growth, migration, tube formation, and levels of lipid peroxidation-derived malondialdehyde (MDA) was examined. Our results indicate that the treatment of HAECs with aspalatone decreased VEGF-induced cell migration, tube formation, and levels of MDA. Aspalatone also inhibited VEGF-induced decrease in the expression of eNOS and increase in the expression of iNOS, ICAM-1, and VCAM-1. Aspalatone also prevented the VEGF-induced adhesion of monocytes to endothelial cells. Furthermore, aspalatone also prevented VEGF-induced release of inflammatory markers such as Angiopoietin-2, Leptin, EGF, G-CSF, HB-EGF, and HGF in HAECs. Thus, our results suggest that aspalatone could be used to prevent endothelial dysfunction, an important process in the pathophysiology of cardiovascular diseases.

Original languageEnglish (US)
Article number2769347
JournalOxidative Medicine and Cellular Longevity
Volume2017
DOIs
StatePublished - 2017

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Endothelial cells
Vascular Endothelial Growth Factor A
Lipid Peroxidation
Endothelial Cells
Lipids
Malondialdehyde
Cell Movement
Angiopoietin-2
aspalatone
Fibrinolytic Agents
Vascular Cell Adhesion Molecule-1
Cell growth
Granulocyte Colony-Stimulating Factor
Intercellular Adhesion Molecule-1
Leptin
Epidermal Growth Factor
Monocytes
Anti-Inflammatory Agents
Cardiovascular Diseases
Adhesion

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Cell Biology

Cite this

Aspalatone Prevents VEGF-Induced Lipid Peroxidation, Migration, Tube Formation, and Dysfunction of Human Aortic Endothelial Cells. / Sonowal, Himangshu; Pal, Pabitra B.; Shukla, Kirtikar; Ramana, Kota.

In: Oxidative Medicine and Cellular Longevity, Vol. 2017, 2769347, 2017.

Research output: Contribution to journalArticle

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