Aspirin induces hepatoma-derived cell apoptosis via a hydrogen peroxide-dependent pathway

Demidmaa Tuvdendorj; Makoto Oketani; Ryuji Ikeda; Kazunori Kohara; Yasuzi Komorizono; Kazuaki Ishibashi; Namid Munkhtuvshin; Terukatsu Arima

doi:10.1016/S1386-6346(03)00003-2

Aspirin induces hepatoma-derived cell apoptosis via a hydrogen peroxide-dependent pathway

Demidmaa Tuvdendorj, Makoto Oketani, Ryuji Ikeda, Kazunori Kohara, Yasuzi Komorizono, Kazuaki Ishibashi, Namid Munkhtuvshin, Terukatsu Arima

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Here we studied whether aspirin (ASA) has any influence on viability of human hepatoma-derived SKHep-1 cells and whether hydrogen peroxide (H₂O₂) has any relation with this effect. ASA inhibited SKHep-1 cell proliferation dose- and time-dependently. Intracellular H₂O₂ increased as early as 15 min after ASA supplementation. Cellular apoptosis correlated with an increase in intracellular H₂O₂ level. Moreover, in the presence of a catalase inhibitor-aminotriazol, ASA showed more apoptotic effect on SKHep-1 cells with increasing intracellular H₂O₂ level. In conclusion, the present results shows that ASA induced SKHep-1 cell apoptosis has a relation with an early increase in intracellular H₂O₂ level and catalase inhibitor synergizes to induce this process.

Original language	English (US)
Pages (from-to)	47-54
Number of pages	8
Journal	Hepatology Research
Volume	26
Issue number	1
DOIs	https://doi.org/10.1016/S1386-6346(03)00003-2
State	Published - May 1 2003
Externally published	Yes

Keywords

Acetylsalicylic acid
Aminotriazol
Hepatocellular carcinoma
Hydrogen peroxide
SKHep-1

ASJC Scopus subject areas

Hepatology
Infectious Diseases

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10.1016/S1386-6346(03)00003-2

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title = "Aspirin induces hepatoma-derived cell apoptosis via a hydrogen peroxide-dependent pathway",

abstract = "Here we studied whether aspirin (ASA) has any influence on viability of human hepatoma-derived SKHep-1 cells and whether hydrogen peroxide (H2O2) has any relation with this effect. ASA inhibited SKHep-1 cell proliferation dose- and time-dependently. Intracellular H2O2 increased as early as 15 min after ASA supplementation. Cellular apoptosis correlated with an increase in intracellular H2O2 level. Moreover, in the presence of a catalase inhibitor-aminotriazol, ASA showed more apoptotic effect on SKHep-1 cells with increasing intracellular H2O2 level. In conclusion, the present results shows that ASA induced SKHep-1 cell apoptosis has a relation with an early increase in intracellular H2O2 level and catalase inhibitor synergizes to induce this process.",

keywords = "Acetylsalicylic acid, Aminotriazol, Hepatocellular carcinoma, Hydrogen peroxide, SKHep-1",

author = "Demidmaa Tuvdendorj and Makoto Oketani and Ryuji Ikeda and Kazunori Kohara and Yasuzi Komorizono and Kazuaki Ishibashi and Namid Munkhtuvshin and Terukatsu Arima",

year = "2003",

month = may,

day = "1",

doi = "10.1016/S1386-6346(03)00003-2",

language = "English (US)",

volume = "26",

pages = "47--54",

journal = "Hepatology Research",

issn = "1386-6346",

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TY - JOUR

T1 - Aspirin induces hepatoma-derived cell apoptosis via a hydrogen peroxide-dependent pathway

AU - Tuvdendorj, Demidmaa

AU - Oketani, Makoto

AU - Ikeda, Ryuji

AU - Kohara, Kazunori

AU - Komorizono, Yasuzi

AU - Ishibashi, Kazuaki

AU - Munkhtuvshin, Namid

AU - Arima, Terukatsu

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Here we studied whether aspirin (ASA) has any influence on viability of human hepatoma-derived SKHep-1 cells and whether hydrogen peroxide (H2O2) has any relation with this effect. ASA inhibited SKHep-1 cell proliferation dose- and time-dependently. Intracellular H2O2 increased as early as 15 min after ASA supplementation. Cellular apoptosis correlated with an increase in intracellular H2O2 level. Moreover, in the presence of a catalase inhibitor-aminotriazol, ASA showed more apoptotic effect on SKHep-1 cells with increasing intracellular H2O2 level. In conclusion, the present results shows that ASA induced SKHep-1 cell apoptosis has a relation with an early increase in intracellular H2O2 level and catalase inhibitor synergizes to induce this process.

AB - Here we studied whether aspirin (ASA) has any influence on viability of human hepatoma-derived SKHep-1 cells and whether hydrogen peroxide (H2O2) has any relation with this effect. ASA inhibited SKHep-1 cell proliferation dose- and time-dependently. Intracellular H2O2 increased as early as 15 min after ASA supplementation. Cellular apoptosis correlated with an increase in intracellular H2O2 level. Moreover, in the presence of a catalase inhibitor-aminotriazol, ASA showed more apoptotic effect on SKHep-1 cells with increasing intracellular H2O2 level. In conclusion, the present results shows that ASA induced SKHep-1 cell apoptosis has a relation with an early increase in intracellular H2O2 level and catalase inhibitor synergizes to induce this process.

KW - Acetylsalicylic acid

KW - Aminotriazol

KW - Hepatocellular carcinoma

KW - Hydrogen peroxide

KW - SKHep-1

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U2 - 10.1016/S1386-6346(03)00003-2

DO - 10.1016/S1386-6346(03)00003-2

M3 - Article

AN - SCOPUS:0042092010

SN - 1386-6346

VL - 26

SP - 47

EP - 54

JO - Hepatology Research

JF - Hepatology Research

IS - 1

ER -

Aspirin induces hepatoma-derived cell apoptosis via a hydrogen peroxide-dependent pathway

Abstract

Keywords

ASJC Scopus subject areas

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