Assay of defective-interfering Semliki forest virus by the inhibition of synthesis of virus-specified RNAs

A. D.T. Barrett, C. F. Crouch, N. J. Dimmock

Research output: Contribution to journalArticle

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Abstract

We describe a simple, rapid and reproducible assay for defective-interfering Semliki Forest virus (DI SFV) which is based on the inhibition of synthesis of virus-specified RNAs in SFV-infected cells. Using the assay, we have been able to show that DI virus is generated by a single passage in baby hamster kidney (BHK) cells in an inoculum which contained no detectable DI virus and we have calculated the u.v. target size of the interfering activity.

Original languageEnglish (US)
Pages (from-to)273-280
Number of pages8
JournalJournal of General Virology
Volume54
Issue number2
DOIs
StatePublished - Jan 1 1981
Externally publishedYes

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ASJC Scopus subject areas

  • Virology

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