Abstract
We describe a simple, rapid and reproducible assay for defective-interfering Semliki Forest virus (DI SFV) which is based on the inhibition of synthesis of virus-specified RNAs in SFV-infected cells. Using the assay, we have been able to show that DI virus is generated by a single passage in baby hamster kidney (BHK) cells in an inoculum which contained no detectable DI virus and we have calculated the u.v. target size of the interfering activity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 273-280 |
| Number of pages | 8 |
| Journal | Journal of General Virology |
| Volume | 54 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1981 |
| Externally published | Yes |
ASJC Scopus subject areas
- Virology