Assaying structural changes after nerve damage, an essay on quantitative morphology

R. E. Coggeshall

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Morphologic changes, such as cell loss or sprouting after nerve lesions, presumably have an important bearing on chronic pain states. Accordingly, unbiased estimates of such things as cell or synapse numbers are necessary to evaluate these difficulties. Basic principles for obtaining such estimates are in dispute, however, so the present paper is an essay on these techniques with the aim of making it easier for nondevotees of these methods to make accurate assessments when such data are reported. The basic points are that, with the exception of serial reconstructions, which are very inefficient, older techniques to determine cell and synapse numbers from histological sections make geometric assumptions that are almost never verified or quantified. Accordingly, the geometrical biases that arise from these assumptions are not assessed. Fortunately, recent stereological techniques do not depend on these assumptions and so are free from the above biases. As such, these represent a technological advance. It must be remembered, however, that all methods are subject to potential observational, methodological and sampling biases.

Original languageEnglish (US)
JournalPain
Volume82
Issue numberSUPPL. 6
DOIs
StatePublished - 1999

Fingerprint

Synapses
Dissent and Disputes
Selection Bias
Chronic Pain
Cell Count

Keywords

  • Pat Wall
  • Stereology
  • Unbiased counting

ASJC Scopus subject areas

  • Clinical Neurology
  • Psychiatry and Mental health
  • Neurology
  • Neuroscience(all)
  • Pharmacology
  • Clinical Psychology

Cite this

Assaying structural changes after nerve damage, an essay on quantitative morphology. / Coggeshall, R. E.

In: Pain, Vol. 82, No. SUPPL. 6, 1999.

Research output: Contribution to journalArticle

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