Assays with recombinant soluble isoforms of DC-SIGN, a dengue virus ligand, show variation in their ability to bind to mannose residues

Lailah Horácio Sales Pereira; Thaís Paiva Porto de Souza; Vidyleison Neves Camargos; Leandro Augusto de Oliveira Barbosa; Alex Guterres Taranto; Moacyr Comar Junior; Hérica de Lima Santos; Débora de Oliveira Lopes; Jaqueline Maria Siqueira Ferreira; Luciana Lara dos Santos

doi:10.1007/s00705-019-04377-9

Assays with recombinant soluble isoforms of DC-SIGN, a dengue virus ligand, show variation in their ability to bind to mannose residues

Lailah Horácio Sales Pereira, Thaís Paiva Porto de Souza, Vidyleison Neves Camargos, Leandro Augusto de Oliveira Barbosa, Alex Guterres Taranto, Moacyr Comar Junior, Hérica de Lima Santos, Débora de Oliveira Lopes, Jaqueline Maria Siqueira Ferreira, Luciana Lara dos Santos

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1 Scopus citations

Abstract

The DC-SIGN glycoprotein is responsible for the initial adhesion of dengue virus (DENV) to immune cells by the carbohydrate recognition domain (CRD). There are thirteen soluble and membrane-bound DC-SIGN isoforms, but the role of soluble isoforms in the DENV internalization process is not known. Five isoforms with an altered or absent CRD were identified, and three different soluble isoforms were used to confirm the interactions with mannose residues. The results show the loss of binding ability of one soluble isoform and binding ability of two of them. All of them will be used to verify their role in the DENV internalization process.

Original language	English (US)
Pages (from-to)	2793-2797
Number of pages	5
Journal	Archives of virology
Volume	164
Issue number	11
DOIs	https://doi.org/10.1007/s00705-019-04377-9
State	Published - Nov 1 2019
Externally published	Yes

ASJC Scopus subject areas

Virology

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Pereira, L. H. S., de Souza, T. P. P., Camargos, V. N., de Oliveira Barbosa, L. A., Taranto, A. G., Junior, M. C., de Lima Santos, H., de Oliveira Lopes, D., Ferreira, J. M. S., & dos Santos, L. L. (2019). Assays with recombinant soluble isoforms of DC-SIGN, a dengue virus ligand, show variation in their ability to bind to mannose residues. Archives of virology, 164(11), 2793-2797. https://doi.org/10.1007/s00705-019-04377-9

Assays with recombinant soluble isoforms of DC-SIGN, a dengue virus ligand, show variation in their ability to bind to mannose residues. / Pereira, Lailah Horácio Sales; de Souza, Thaís Paiva Porto; Camargos, Vidyleison Neves et al.
In: Archives of virology, Vol. 164, No. 11, 01.11.2019, p. 2793-2797.

Research output: Contribution to journal › Article › peer-review

Pereira, LHS, de Souza, TPP, Camargos, VN, de Oliveira Barbosa, LA, Taranto, AG, Junior, MC, de Lima Santos, H, de Oliveira Lopes, D, Ferreira, JMS & dos Santos, LL 2019, 'Assays with recombinant soluble isoforms of DC-SIGN, a dengue virus ligand, show variation in their ability to bind to mannose residues', Archives of virology, vol. 164, no. 11, pp. 2793-2797. https://doi.org/10.1007/s00705-019-04377-9

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abstract = "The DC-SIGN glycoprotein is responsible for the initial adhesion of dengue virus (DENV) to immune cells by the carbohydrate recognition domain (CRD). There are thirteen soluble and membrane-bound DC-SIGN isoforms, but the role of soluble isoforms in the DENV internalization process is not known. Five isoforms with an altered or absent CRD were identified, and three different soluble isoforms were used to confirm the interactions with mannose residues. The results show the loss of binding ability of one soluble isoform and binding ability of two of them. All of them will be used to verify their role in the DENV internalization process.",

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AU - Camargos, Vidyleison Neves

AU - de Oliveira Barbosa, Leandro Augusto

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AB - The DC-SIGN glycoprotein is responsible for the initial adhesion of dengue virus (DENV) to immune cells by the carbohydrate recognition domain (CRD). There are thirteen soluble and membrane-bound DC-SIGN isoforms, but the role of soluble isoforms in the DENV internalization process is not known. Five isoforms with an altered or absent CRD were identified, and three different soluble isoforms were used to confirm the interactions with mannose residues. The results show the loss of binding ability of one soluble isoform and binding ability of two of them. All of them will be used to verify their role in the DENV internalization process.

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Assays with recombinant soluble isoforms of DC-SIGN, a dengue virus ligand, show variation in their ability to bind to mannose residues

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