Assembled coronavirus from complementation of two defective interfering RNAs

Kyongmin Hwang Kim, Krishna Narayanan, Shinji Makino

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In the presence of an RNA- temperature-sensitive (ts) mutant helper virus, two coronavirus mouse hepatitis virus (MHV) defective interfering (DI) RNAs complemented each other, resulting in the assembly of MHV particles; we used this ability to complement as a means to study coronavirus assembly. One of the two DI RNAs was DIssA, a naturally occurring self-replicating DI RNA encoding N protein and the gene 1 proteins that encode RNA polymerase function; DIssA supports the replication and transcription of other non- self-replicating DI RNAs. The other DI was a genetically engineered DI RNA that encoded sM and M proteins. Coinfection of these two DIs at the nonpermissive temperature for the ts helper virus resulted in replication and transcription of both DI RNAs but not in synthesis of the helper virus RNAs. MHV particles containing DI RNAs, N protein, and M protein, all of which were exclusively derived from the two DI RNAs, were released from the coinfected cells; the amount of sM protein was below the limits of detection. Analyses of DI RNAs with mutations in the two envelope protein genes demonstrated that M and sM proteins appeared to be required for assembly and release of MHV particles that contained DI RNAs and N protein, while S protein was not required for assembly and release of MHV particles.

Original languageEnglish (US)
Pages (from-to)3922-3931
Number of pages10
JournalJournal of Virology
Volume71
Issue number5
StatePublished - May 1997
Externally publishedYes

Fingerprint

Coronavirus
Coronavirinae
Murine hepatitis virus
RNA
virion
Helper Viruses
proteins
Virion
Proteins
Temperature
transcription (genetics)
temperature
DNA-directed RNA polymerase
virus replication
Protein S
mixed infection
DNA-Directed RNA Polymerases
Coinfection
complement
detection limit

ASJC Scopus subject areas

  • Immunology

Cite this

Assembled coronavirus from complementation of two defective interfering RNAs. / Kim, Kyongmin Hwang; Narayanan, Krishna; Makino, Shinji.

In: Journal of Virology, Vol. 71, No. 5, 05.1997, p. 3922-3931.

Research output: Contribution to journalArticle

Kim, Kyongmin Hwang ; Narayanan, Krishna ; Makino, Shinji. / Assembled coronavirus from complementation of two defective interfering RNAs. In: Journal of Virology. 1997 ; Vol. 71, No. 5. pp. 3922-3931.
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