TY - JOUR
T1 - Assessing incomplete deprotection of microarray oligonucleotides in situ
AU - Dressman, Holly K.
AU - Barley-Maloney, Lise
AU - Rowlette, Laura Leigh
AU - Agris, Paul F.
AU - Garcia-Blanco, Mariano A.
PY - 2006/11
Y1 - 2006/11
N2 - En masse analysis of gene structure and function by array technologies will have a lasting and profound effect on biology and medicine. This impact can be compromised by low quality of probes within arrays, which we show can be caused by incomplete removal of chemical protecting groups. To solve this quality control problem, we present a sensitive, specific and facile method to detect these groups in situ on arrays using monoclonal antibodies and existing instrumentation. Screening of microarrays with these monoclonal antibodies should guide the consideration given to data derived from these and should enhance the accuracy of the results obtained.
AB - En masse analysis of gene structure and function by array technologies will have a lasting and profound effect on biology and medicine. This impact can be compromised by low quality of probes within arrays, which we show can be caused by incomplete removal of chemical protecting groups. To solve this quality control problem, we present a sensitive, specific and facile method to detect these groups in situ on arrays using monoclonal antibodies and existing instrumentation. Screening of microarrays with these monoclonal antibodies should guide the consideration given to data derived from these and should enhance the accuracy of the results obtained.
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U2 - 10.1093/nar/gkl713
DO - 10.1093/nar/gkl713
M3 - Article
C2 - 17020916
AN - SCOPUS:33750991903
SN - 0305-1048
VL - 34
JO - Nucleic acids research
JF - Nucleic acids research
IS - 19
M1 - e131
ER -