Assessment of candidate plant DNA barcodes using the Rutaceae family

Kun Luo, Shi Lin Chen, Ke Li Chen, Jing Yuan Song, Hui Yao, Xinye Ma, Yingjie Zhu, Xiao Hui Pang, Hua Yu, Xi Wen Li, Zhen Liu

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

DNA barcoding is a rapidly developing frontier technology that is gaining worldwide attention. Here, seven regions (psbA-trnH, matK, ycf5, rpoC1, rbcL, ITS2, and ITS) with potential for use as DNA barcodes were tested for their ability to identify 300 samples of 192 species from 72 genera of the family Rutaceae. To evaluate each barcode's utility for species authentication, PCR amplification efficiency, genetic divergence, and barcoding gaps were assessed. We found that the ITS2 region exhibited the highest inter-specific divergence, and that this was significantly higher than the intra-specific variation in the "DNA barcoding gap" assessment and Wilcoxon two-sample tests. The ITS2 locus had the highest identification efficiency among all tested regions. In a previous study, we found that ITS2 was able to discriminate a wide range of plant taxa, and here we confirmed that ITS2 was also able to discriminate a number of closely related species. Therefore, we propose that ITS2 is a promising candidate barcode for plant species identification.

Original languageEnglish (US)
Pages (from-to)701-708
Number of pages8
JournalScience China Life Sciences
Volume53
Issue number6
DOIs
StatePublished - Jul 9 2010
Externally publishedYes

Keywords

  • DNA barcoding
  • Identification
  • ITS2
  • Rutaceae

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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  • Cite this

    Luo, K., Chen, S. L., Chen, K. L., Song, J. Y., Yao, H., Ma, X., Zhu, Y., Pang, X. H., Yu, H., Li, X. W., & Liu, Z. (2010). Assessment of candidate plant DNA barcodes using the Rutaceae family. Science China Life Sciences, 53(6), 701-708. https://doi.org/10.1007/s11427-010-4009-1