Assessment of the efficacy of commercially available and candidate vaccines against a pandemic H1N1 2009 virus

Gary P. Kobinger, Isabelle Meunier, Ami Patel, Stéphane Pillet, Jason Gren, Shane Stebner, Anders Leung, James L. Neufeld, Darwyn Kobasa, Veronika Von Messlling

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


Background. The emergence and global spread of the pandemic H1N1 2009 influenza virus have raised questions regarding the protective effect of available seasonal vaccines and the efficacy of a newly produced matched vaccine. Methods. Ferrets were immunized with the 2008-2009 formulations of commercially available live attenuated (FluMist; Medlmmune) or split-inactivated (Fluviral; GlaxoSmithKline) vaccines, a commercial swine vaccine (FluSure; Pfizer), or a laboratory-produced matched inactivated whole-virus vaccine (A/Mexico/InDRE4487/2009). Adaptive immune responses were monitored, and the animals were challenged with A/Mexico/InDRE4487/2009 after 5 weeks. Results. Only animals that received the swine or matched vaccines developed detectable hemagglutinationinhibiting antibodies against the challenge virus, whereas a T cell response was exclusively detected in animals vaccinated with FluMist. After challenge, all animals had high levels of virus replication in the upper respiratory tract. However, preexisting anti-pandemic H1N1 2009 antibodies resulted in reduced clinical signs and improved survival. Surprisingly, FluMist was associated with a slight increase in mortality and greater lung damage, which correlated with early up-regulation of interleukin-10. Conclusions. The present study demonstrates that a single dose of matched inactivated vaccine confers partial protection against a pandemic H1N1 2009 virus, and it suggests that a higher dose or prime-boost regimen may be required. The consequences of mismatched immunity to influenza merit further investigation.

Original languageEnglish (US)
Pages (from-to)1000-1006
Number of pages7
JournalJournal of Infectious Diseases
Issue number7
StatePublished - Apr 1 2010
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases


Dive into the research topics of 'Assessment of the efficacy of commercially available and candidate vaccines against a pandemic H1N1 2009 virus'. Together they form a unique fingerprint.

Cite this