TY - JOUR
T1 - Association of biomarkers of inflammation and oxidative stress with the risk of chronic kidney disease in Type 2 diabetes mellitus in North Indian population
AU - Gupta, Stuti
AU - Gambhir, Jasvinder K.
AU - Kalra, Op
AU - Gautam, Amar
AU - Shukla, Kirtikar
AU - Mehndiratta, Mohit
AU - Agarwal, Sunil
AU - Shukla, Rimi
N1 - Funding Information:
We sincerely thank Indian Council of Medical Research and University college of medical sciences, New Delhi for providing partial funding for this study. The authors are also thankful to the Department of Biostatistics and Medical Informatics, University College of Medical Sciences, Delhi for statistical analysis.
PY - 2013/11
Y1 - 2013/11
N2 - Chronic kidney disease (CKD) is a major cause of morbidity and mortality worldwide. It results from diverse etiologies, diabetes being a frontrunner amongst them. Type 2 diabetes mellitus (DM) is being increasingly recognized as a proinflammatory state with increased oxidative stress which enormously increases the risk of micro and macro vascular diseases. This study was planned to explore the possible association between tumor necrosis factor-alpha (TNF-α), urinary monocyte chemoattractant protein-1 (uMCP-1), high-sensitivity C-reactive protein (hsCRP) and parameters of oxidative stress in patients with Type 2 diabetes mellitus (DM) and diabetic chronic kidney disease (DM-CKD). Fifty patients each were recruited in DM, DM-CKD and healthy control groups. Plasma TNF-α, hsCRP and uMCP-1 levels as inflammatory mediators were measured by ELISA, reduced glutathione (GSH), ferric reducing ability of plasma (FRAP) as parameters of antioxidant activity and malondialdehyde (MDA) as marker of oxidative stress, were measured spectrophotometrically. Plasma TNF-α, hsCRP and uMCP-1 were significantly higher in DM-CKD compared to DM and healthy controls. Lipid peroxidation, measured as MDA was significantly higher in patients with DM-CKD as compared to patients with DM and healthy controls. Further, antioxidant capacity of blood measured as FRAP and GSH was found to be significantly lower in patients with DM and DM-CKD as compared to healthy controls (p < 0.001). Plasma TNF-α and uMCP-1 showed a significant positive correlation with HbA1c (r = 0.441, 0.643), hsCRP (r = 0.400, 0.584) and MDA (r = 0.423, 0.759) and significant negative correlation with GSH (R = - 0.370, - 0.800) and FRAP (r = - 0.344, - 0.684) Increased inflammatory markers viz. TNF-α, hsCRP and uMCP-1 and markers of oxidative stress i.e. increased MDA and decreased GSH and FRAP in DM-CKD suggest an important role of inflammation and oxidative stress in the pathogenesis of renal damage in diabetic patients.
AB - Chronic kidney disease (CKD) is a major cause of morbidity and mortality worldwide. It results from diverse etiologies, diabetes being a frontrunner amongst them. Type 2 diabetes mellitus (DM) is being increasingly recognized as a proinflammatory state with increased oxidative stress which enormously increases the risk of micro and macro vascular diseases. This study was planned to explore the possible association between tumor necrosis factor-alpha (TNF-α), urinary monocyte chemoattractant protein-1 (uMCP-1), high-sensitivity C-reactive protein (hsCRP) and parameters of oxidative stress in patients with Type 2 diabetes mellitus (DM) and diabetic chronic kidney disease (DM-CKD). Fifty patients each were recruited in DM, DM-CKD and healthy control groups. Plasma TNF-α, hsCRP and uMCP-1 levels as inflammatory mediators were measured by ELISA, reduced glutathione (GSH), ferric reducing ability of plasma (FRAP) as parameters of antioxidant activity and malondialdehyde (MDA) as marker of oxidative stress, were measured spectrophotometrically. Plasma TNF-α, hsCRP and uMCP-1 were significantly higher in DM-CKD compared to DM and healthy controls. Lipid peroxidation, measured as MDA was significantly higher in patients with DM-CKD as compared to patients with DM and healthy controls. Further, antioxidant capacity of blood measured as FRAP and GSH was found to be significantly lower in patients with DM and DM-CKD as compared to healthy controls (p < 0.001). Plasma TNF-α and uMCP-1 showed a significant positive correlation with HbA1c (r = 0.441, 0.643), hsCRP (r = 0.400, 0.584) and MDA (r = 0.423, 0.759) and significant negative correlation with GSH (R = - 0.370, - 0.800) and FRAP (r = - 0.344, - 0.684) Increased inflammatory markers viz. TNF-α, hsCRP and uMCP-1 and markers of oxidative stress i.e. increased MDA and decreased GSH and FRAP in DM-CKD suggest an important role of inflammation and oxidative stress in the pathogenesis of renal damage in diabetic patients.
KW - Chronic kidney disease
KW - Inflammation
KW - Oxidative stress
KW - Type 2 DM
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U2 - 10.1016/j.jdiacomp.2013.07.005
DO - 10.1016/j.jdiacomp.2013.07.005
M3 - Article
C2 - 24012111
AN - SCOPUS:84887071509
SN - 1056-8727
VL - 27
SP - 548
EP - 552
JO - Journal of Diabetes and Its Complications
JF - Journal of Diabetes and Its Complications
IS - 6
ER -