Association of G(IR), a novel 66 kDa GTP-binding placental protein, with insulin receptor

T. K. Varma, J. Johnson, Satish Srivastava

Research output: Contribution to journalArticle

Abstract

Subsequent to the binding of insulin to the insulin receptor (IR), present at the cell surface of all the tissues studied so far, conformational change in IR leads to the activation of IR tyrosine kinase. However, the transducer of the signal from IR to its effector(s) is poorly understood, at best. Although GTP-binding proteins (G-proteins) have been implicated in insulin's metabolic actions, a G-protein that directly interacts with IR has not been identified. In the present study, a novel 66 kDa GTP-binding protein, G(ir), has been isolated and characterized. IR and G(ir) from human placental membrane bound to insulin-Sepharose column. GTP as well as GDP (1 mM) eluted G(ir) from the column and acetate buffer (pH 5.0) eluted both IR and G(ir). Both IR and G(ir) thus eluted, absorbed on the anti-IR-Sepharose column and GTP eluted G(ir). Antibodies against synthetic peptides from GTP-binding and ADP-ribosylation domains of G(zα) and G(i-3α), respectively, cross-reacted with G(ir) at various stages of purification. Insulin-activated IR tyrosine kinase phosphorylated G(ir) which was immunoprecipitated by both the antisera. These studies indicate that IR is complexed with G(ir), which could be one of the G-proteins implicated in insulin's signal transduction.

Original languageEnglish (US)
Pages (from-to)807-817
Number of pages11
JournalBiochemistry and Molecular Biology International
Volume32
Issue number5
StatePublished - 1994

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Pregnancy Proteins
Insulin Receptor
Guanosine Triphosphate
GTP-Binding Proteins
Insulin
Signal transduction
Transducers
Sepharose
Adenosine Diphosphate
Purification
Immune Sera
Signal Transduction
Buffers
Acetates
Chemical activation
Tissue
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Association of G(IR), a novel 66 kDa GTP-binding placental protein, with insulin receptor. / Varma, T. K.; Johnson, J.; Srivastava, Satish.

In: Biochemistry and Molecular Biology International, Vol. 32, No. 5, 1994, p. 807-817.

Research output: Contribution to journalArticle

Varma, T. K. ; Johnson, J. ; Srivastava, Satish. / Association of G(IR), a novel 66 kDa GTP-binding placental protein, with insulin receptor. In: Biochemistry and Molecular Biology International. 1994 ; Vol. 32, No. 5. pp. 807-817.
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