Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories

Agnieszka D'Antonio-Chronowska; Margaret K.R. Donovan; William W. Young Greenwald; Jennifer Phuong Nguyen; Kyohei Fujita; Sherin Hashem; Hiroko Matsui; Francesca Soncin; Mana Parast; Michelle C. Ward; Florence Coulet; Erin N. Smith; Eric Adler; Matteo D'Antonio; Kelly A. Frazer

doi:10.1016/j.stemcr.2019.09.011

Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories

Agnieszka D'Antonio-Chronowska, Margaret K.R. Donovan, William W. Young Greenwald, Jennifer Phuong Nguyen, Kyohei Fujita, Sherin Hashem, Hiroko Matsui, Francesca Soncin, Mana Parast, Michelle C. Ward, Florence Coulet, Erin N. Smith, Eric Adler, Matteo D'Antonio, Kelly A. Frazer

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

In this article, D'Antonio-Chronowska, Donovan and colleagues differentiated 191 iPSC lines to generate iPSC-derived cardiovascular progenitor cells (iPSC-CVPCs). The iPSC-CVPC samples showed cellular heterogeneity due to varying fractions of cardiomyocytes (CMs) and epicardium-derived cells (EPDCs). Donor sex and expression levels of signature genes in iPSCs played a role in the relative proportions of CMs and EPDCs present in the derived CVPCs.

Original language	English (US)
Pages (from-to)	924-938
Number of pages	15
Journal	Stem Cell Reports
Volume	13
Issue number	5
DOIs	https://doi.org/10.1016/j.stemcr.2019.09.011
State	Published - Nov 12 2019
Externally published	Yes

Keywords

X chromosome erosion
X chromosome inactivation
iPSC
iPSC differentiation
iPSC-derived cardiomyocytes
iPSC-derived cardiovascular progenitor cells
iPSC-derived epicardium
scRNA-seq
single-cell transcriptomics

ASJC Scopus subject areas

Biochemistry
Genetics
Developmental Biology
Cell Biology

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10.1016/j.stemcr.2019.09.011

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D'Antonio-Chronowska, A., Donovan, M. K. R., Young Greenwald, W. W., Nguyen, J. P., Fujita, K., Hashem, S., Matsui, H., Soncin, F., Parast, M., Ward, M. C., Coulet, F., Smith, E. N., Adler, E., D'Antonio, M., & Frazer, K. A. (2019). Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories. Stem Cell Reports, 13(5), 924-938. https://doi.org/10.1016/j.stemcr.2019.09.011

D'Antonio-Chronowska, A, Donovan, MKR, Young Greenwald, WW, Nguyen, JP, Fujita, K, Hashem, S, Matsui, H, Soncin, F, Parast, M, Ward, MC, Coulet, F, Smith, EN, Adler, E, D'Antonio, M & Frazer, KA 2019, 'Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories', Stem Cell Reports, vol. 13, no. 5, pp. 924-938. https://doi.org/10.1016/j.stemcr.2019.09.011

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abstract = "In this article, D'Antonio-Chronowska, Donovan and colleagues differentiated 191 iPSC lines to generate iPSC-derived cardiovascular progenitor cells (iPSC-CVPCs). The iPSC-CVPC samples showed cellular heterogeneity due to varying fractions of cardiomyocytes (CMs) and epicardium-derived cells (EPDCs). Donor sex and expression levels of signature genes in iPSCs played a role in the relative proportions of CMs and EPDCs present in the derived CVPCs.",

keywords = "X chromosome erosion, X chromosome inactivation, iPSC, iPSC differentiation, iPSC-derived cardiomyocytes, iPSC-derived cardiovascular progenitor cells, iPSC-derived epicardium, scRNA-seq, single-cell transcriptomics",

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doi = "10.1016/j.stemcr.2019.09.011",

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AU - Young Greenwald, William W.

AU - Nguyen, Jennifer Phuong

AU - Fujita, Kyohei

AU - Hashem, Sherin

AU - Matsui, Hiroko

AU - Soncin, Francesca

AU - Parast, Mana

AU - Ward, Michelle C.

AU - Coulet, Florence

AU - Smith, Erin N.

AU - Adler, Eric

AU - D'Antonio, Matteo

AU - Frazer, Kelly A.

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KW - X chromosome inactivation

KW - iPSC

KW - iPSC differentiation

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KW - iPSC-derived epicardium

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Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories

Abstract

Keywords

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