Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories

Agnieszka D'Antonio-Chronowska, Margaret K.R. Donovan, William W. Young Greenwald, Jennifer Phuong Nguyen, Kyohei Fujita, Sherin Hashem, Hiroko Matsui, Francesca Soncin, Mana Parast, Michelle C. Ward, Florence Coulet, Erin N. Smith, Eric Adler, Matteo D'Antonio, Kelly A. Frazer

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

In this article, D'Antonio-Chronowska, Donovan and colleagues differentiated 191 iPSC lines to generate iPSC-derived cardiovascular progenitor cells (iPSC-CVPCs). The iPSC-CVPC samples showed cellular heterogeneity due to varying fractions of cardiomyocytes (CMs) and epicardium-derived cells (EPDCs). Donor sex and expression levels of signature genes in iPSCs played a role in the relative proportions of CMs and EPDCs present in the derived CVPCs.

Original languageEnglish (US)
Pages (from-to)924-938
Number of pages15
JournalStem Cell Reports
Volume13
Issue number5
DOIs
StatePublished - Nov 12 2019

Keywords

  • X chromosome erosion
  • X chromosome inactivation
  • iPSC
  • iPSC differentiation
  • iPSC-derived cardiomyocytes
  • iPSC-derived cardiovascular progenitor cells
  • iPSC-derived epicardium
  • scRNA-seq
  • single-cell transcriptomics

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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    D'Antonio-Chronowska, A., Donovan, M. K. R., Young Greenwald, W. W., Nguyen, J. P., Fujita, K., Hashem, S., Matsui, H., Soncin, F., Parast, M., Ward, M. C., Coulet, F., Smith, E. N., Adler, E., D'Antonio, M., & Frazer, K. A. (2019). Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories. Stem Cell Reports, 13(5), 924-938. https://doi.org/10.1016/j.stemcr.2019.09.011