Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories

Agnieszka D'Antonio-Chronowska, Margaret K.R. Donovan, William W. Young Greenwald, Jennifer Phuong Nguyen, Kyohei Fujita, Sherin Hashem, Hiroko Matsui, Francesca Soncin, Mana Parast, Michelle C. Ward, Florence Coulet, Erin N. Smith, Eric Adler, Matteo D'Antonio, Kelly A. Frazer

Abstract

In this article, D'Antonio-Chronowska, Donovan and colleagues differentiated 191 iPSC lines to generate iPSC-derived cardiovascular progenitor cells (iPSC-CVPCs). The iPSC-CVPC samples showed cellular heterogeneity due to varying fractions of cardiomyocytes (CMs) and epicardium-derived cells (EPDCs). Donor sex and expression levels of signature genes in iPSCs played a role in the relative proportions of CMs and EPDCs present in the derived CVPCs.

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D'Antonio-Chronowska, A., Donovan, M. K. R., Young Greenwald, W. W., Nguyen, J. P., Fujita, K., Hashem, S., Matsui, H., Soncin, F., Parast, M., Ward, M. C., Coulet, F., Smith, E. N., Adler, E., D'Antonio, M., & Frazer, K. A. (2019). Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories. Stem Cell Reports, 13(5), 924-938. https://doi.org/10.1016/j.stemcr.2019.09.011