Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories

Agnieszka D'Antonio-Chronowska; Margaret K.R. Donovan; William W. Young Greenwald; Jennifer Phuong Nguyen; Kyohei Fujita; Sherin Hashem; Hiroko Matsui; Francesca Soncin; Mana Parast; Michelle C. Ward; Florence Coulet; Erin N. Smith; Eric Adler; Matteo D'Antonio; Kelly A. Frazer

doi:10.1016/j.stemcr.2019.09.011

Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories

Agnieszka D'Antonio-Chronowska
, Margaret K.R. Donovan
, William W. Young Greenwald
, Jennifer Phuong Nguyen
, Kyohei Fujita
, Sherin Hashem
, Hiroko Matsui
, Francesca Soncin
, Mana Parast
, Michelle C. Ward
, Florence Coulet
, Erin N. Smith
, Eric Adler
, Matteo D'Antonio
, Kelly A. Frazer

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

In this article, D'Antonio-Chronowska, Donovan and colleagues differentiated 191 iPSC lines to generate iPSC-derived cardiovascular progenitor cells (iPSC-CVPCs). The iPSC-CVPC samples showed cellular heterogeneity due to varying fractions of cardiomyocytes (CMs) and epicardium-derived cells (EPDCs). Donor sex and expression levels of signature genes in iPSCs played a role in the relative proportions of CMs and EPDCs present in the derived CVPCs.

Original language	English (US)
Pages (from-to)	924-938
Number of pages	15
Journal	Stem Cell Reports
Volume	13
Issue number	5
DOIs	https://doi.org/10.1016/j.stemcr.2019.09.011
State	Published - Nov 12 2019
Externally published	Yes

Keywords

X chromosome erosion
X chromosome inactivation
iPSC
iPSC differentiation
iPSC-derived cardiomyocytes
iPSC-derived cardiovascular progenitor cells
iPSC-derived epicardium
scRNA-seq
single-cell transcriptomics

ASJC Scopus subject areas

Biochemistry
Genetics
Developmental Biology
Cell Biology

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10.1016/j.stemcr.2019.09.011

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D'Antonio-Chronowska, A., Donovan, M. K. R., Young Greenwald, W. W., Nguyen, J. P., Fujita, K., Hashem, S., Matsui, H., Soncin, F., Parast, M., Ward, M. C., Coulet, F., Smith, E. N., Adler, E., D'Antonio, M., & Frazer, K. A. (2019). Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories. Stem Cell Reports, 13(5), 924-938. https://doi.org/10.1016/j.stemcr.2019.09.011

D'Antonio-Chronowska, A, Donovan, MKR, Young Greenwald, WW, Nguyen, JP, Fujita, K, Hashem, S, Matsui, H, Soncin, F, Parast, M, Ward, MC, Coulet, F, Smith, EN, Adler, E, D'Antonio, M & Frazer, KA 2019, 'Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories', Stem Cell Reports, vol. 13, no. 5, pp. 924-938. https://doi.org/10.1016/j.stemcr.2019.09.011

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abstract = "In this article, D'Antonio-Chronowska, Donovan and colleagues differentiated 191 iPSC lines to generate iPSC-derived cardiovascular progenitor cells (iPSC-CVPCs). The iPSC-CVPC samples showed cellular heterogeneity due to varying fractions of cardiomyocytes (CMs) and epicardium-derived cells (EPDCs). Donor sex and expression levels of signature genes in iPSCs played a role in the relative proportions of CMs and EPDCs present in the derived CVPCs.",

keywords = "X chromosome erosion, X chromosome inactivation, iPSC, iPSC differentiation, iPSC-derived cardiomyocytes, iPSC-derived cardiovascular progenitor cells, iPSC-derived epicardium, scRNA-seq, single-cell transcriptomics",

author = "Agnieszka D'Antonio-Chronowska and Donovan, \{Margaret K.R.\} and \{Young Greenwald\}, \{William W.\} and Nguyen, \{Jennifer Phuong\} and Kyohei Fujita and Sherin Hashem and Hiroko Matsui and Francesca Soncin and Mana Parast and Ward, \{Michelle C.\} and Florence Coulet and Smith, \{Erin N.\} and Eric Adler and Matteo D'Antonio and Frazer, \{Kelly A.\}",

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doi = "10.1016/j.stemcr.2019.09.011",

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AU - Donovan, Margaret K.R.

AU - Young Greenwald, William W.

AU - Nguyen, Jennifer Phuong

AU - Fujita, Kyohei

AU - Hashem, Sherin

AU - Matsui, Hiroko

AU - Soncin, Francesca

AU - Parast, Mana

AU - Ward, Michelle C.

AU - Coulet, Florence

AU - Smith, Erin N.

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AU - D'Antonio, Matteo

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AB - In this article, D'Antonio-Chronowska, Donovan and colleagues differentiated 191 iPSC lines to generate iPSC-derived cardiovascular progenitor cells (iPSC-CVPCs). The iPSC-CVPC samples showed cellular heterogeneity due to varying fractions of cardiomyocytes (CMs) and epicardium-derived cells (EPDCs). Donor sex and expression levels of signature genes in iPSCs played a role in the relative proportions of CMs and EPDCs present in the derived CVPCs.

KW - X chromosome erosion

KW - X chromosome inactivation

KW - iPSC

KW - iPSC differentiation

KW - iPSC-derived cardiomyocytes

KW - iPSC-derived cardiovascular progenitor cells

KW - iPSC-derived epicardium

KW - scRNA-seq

KW - single-cell transcriptomics

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Association of Human iPSC Gene Signatures and X Chromosome Dosage with Two Distinct Cardiac Differentiation Trajectories

Abstract

Keywords

ASJC Scopus subject areas

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