Association of hyperglycemia mediated increased advanced glycation and erythrocyte antioxidant enzyme activity in different stages of diabetic retinopathy

Subhadip Choudhuri, Deep Dutta, Imran H. Chowdhury, Bhaskar Mitra, Aditi Sen, Lakshmi K. Mandal, Satinath Mukhopadhyay, Basudev Bhattacharya

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Aim: This study aimed to evaluate whether hyperglycemia mediated increased formation of advanced glycation end products (AGEs) was associated with erythrocyte antioxidant enzyme activity in subjects with different stages of diabetic retinopathy (DR). Methods: Serum level of AGEs was determined by enzyme linked immunosorbent assay. Erythrocyte superoxide dismutase (SOD), glutathione reductase (GR) and catalase activity were estimated by enzymatic reaction based spectrophotometric assay in patients with type 2 diabetes with proliferative diabetic retinopathy (PDR), non-proliferative diabetic retinopathy (NPDR) and no retinopathy (DNR) and also in healthy non-diabetic controls (HC). Result: Erythrocyte SOD and GR activity was significantly lower among NPDR (p= 0.024, 0.0017, respectively) and PDR (p= 0.0003, 0.0001, respectively) subjects compared with DNR individuals. A significant inverse correlation was observed between serum AGEs and erythrocyte SOD or GR activity in DNR (p= 0.0019; r= -0.3033, p= 0.0021; r= -0.3015, respectively), NPDR (p= 0.0001; r= -0.4602, p= 0.0003; r= -0.4161, respectively), and PDR (p<. 0.0001; r= -0.6753, p<. 0.0001; r= -0.5854, respectively) individuals. Conclusion: Poor glycemia may be the key factor enhancing AGE formation, which may be associated with lower erythrocyte SOD and GR activity along with increased catalase activity in DR.

Original languageEnglish (US)
Pages (from-to)376-384
Number of pages9
JournalDiabetes Research and Clinical Practice
Volume100
Issue number3
DOIs
StatePublished - Jun 2013
Externally publishedYes

Keywords

  • Advanced glycation end products
  • Catalase
  • Glutathione reductase
  • Non-proliferative diabetic retinopathy
  • Proliferative diabetic retinopathy
  • Superoxide dismutase

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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