Association of the TLX-2 homeodomain and 14-3-3η signaling proteins

Shao-Jun Tang, Ting Chung Suen, Roderick R. McInnes, Manuel Buchwald

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Homeodomain proteins play important roles in various developmental processes, and their functions are modulated by polypeptide cofactors. Here we report that both in vitro and in vivo, 14-3-3η is associated with the TLX-2 homeodomain transcription factor that is required for mouse embryogenesis. Expression of 14-3-3η shifts the predominant localization of TLX-2 in COS cells from the cytoplasm to the nucleus. Tlx-2 and 14-3-3η are expressed in the developing peripheral nervous system with spatially and temporally overlapping patterns, and they are also coexpressed in PC12 cells. Increased expression of either gene by transfection considerably inhibited nerve growth factor-induced neurite outgrowth of PC12 cells, and cotransfection of both genes led to a synergistic effect of suppression. These findings define 14-3-3η as a functional modulator of the TLX-2 homeodomain transcription factor and suggest that the in vivo function of TLX-2 in neural differentiation is likely regulated by signaling mediated by 14-3-3η.

Original languageEnglish (US)
Pages (from-to)25356-25363
Number of pages8
JournalJournal of Biological Chemistry
Volume273
Issue number39
DOIs
StatePublished - Sep 25 1998
Externally publishedYes

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14-3-3 Proteins
PC12 Cells
Transcription Factors
Genes
Homeodomain Proteins
COS Cells
Peripheral Nervous System
Neurology
Nerve Growth Factor
Modulators
Embryonic Development
Transfection
Cytoplasm
Proteins
Gene Expression
Peptides
In Vitro Techniques
Neuronal Outgrowth

ASJC Scopus subject areas

  • Biochemistry

Cite this

Association of the TLX-2 homeodomain and 14-3-3η signaling proteins. / Tang, Shao-Jun; Suen, Ting Chung; McInnes, Roderick R.; Buchwald, Manuel.

In: Journal of Biological Chemistry, Vol. 273, No. 39, 25.09.1998, p. 25356-25363.

Research output: Contribution to journalArticle

Tang, Shao-Jun ; Suen, Ting Chung ; McInnes, Roderick R. ; Buchwald, Manuel. / Association of the TLX-2 homeodomain and 14-3-3η signaling proteins. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 39. pp. 25356-25363.
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