TY - JOUR
T1 - Association of three small GTP-binding proteins with cholinergic synaptic vesicles
AU - Volknandt, Walter
AU - Pevsne, Jonathan
AU - Elferink, Lisa A.
AU - Scheller, Richard H.
N1 - Funding Information:
Acknowledgements: We thank Dr. J. Schilling and the Stanford Protein and Nucleic Acid Facility for amino acid sequencing, M. SchlPfer and A. Winter for experimental assistence,a nd Dr. H. Zimmermann for dlscussion. This work was supported by HHMI and NIMH, SFB 169 anda collaboratirvees earcghra nfto r W.V. from the NATO.
PY - 1993/2/8
Y1 - 1993/2/8
N2 - Several small (low molecular weight) GTP-binding proteins are associated with cholinergic synaptic vesicles derived from the electric organ of electric ray. Using GTP overlay techniques and direct micro sequencing we analyzed the association of small GTP-binding proteins with synaptic vesicles. Both experimental procedures revealed the specific occurrence of multiple small GTP-binding proteins with this organelle. Moreover, direct amino acid sequence analysis assigned at least three different small GTP-binding proteins, ora3, o-ral and o-rab3, to the vesicular compartment. Furthermore, the data reflect the relative abundance of these three proteins on the vesicle membrane, thereby demonstrating the predominant occurrence of o-rab3, the only exclusively synaptic vesicle specific small GTP-binding protein.
AB - Several small (low molecular weight) GTP-binding proteins are associated with cholinergic synaptic vesicles derived from the electric organ of electric ray. Using GTP overlay techniques and direct micro sequencing we analyzed the association of small GTP-binding proteins with synaptic vesicles. Both experimental procedures revealed the specific occurrence of multiple small GTP-binding proteins with this organelle. Moreover, direct amino acid sequence analysis assigned at least three different small GTP-binding proteins, ora3, o-ral and o-rab3, to the vesicular compartment. Furthermore, the data reflect the relative abundance of these three proteins on the vesicle membrane, thereby demonstrating the predominant occurrence of o-rab3, the only exclusively synaptic vesicle specific small GTP-binding protein.
KW - Cholinergic synaptic vesicle
KW - Electric ray electric organ
KW - GTP-binding protein
UR - http://www.scopus.com/inward/record.url?scp=0027533461&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027533461&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(93)81490-Q
DO - 10.1016/0014-5793(93)81490-Q
M3 - Article
C2 - 8428634
AN - SCOPUS:0027533461
VL - 317
SP - 53
EP - 56
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -