Association of three small GTP-binding proteins with cholinergic synaptic vesicles

Walter Volknandt, Jonathan Pevsne, Lisa Elferink, Richard H. Scheller

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Several small (low molecular weight) GTP-binding proteins are associated with cholinergic synaptic vesicles derived from the electric organ of electric ray. Using GTP overlay techniques and direct micro sequencing we analyzed the association of small GTP-binding proteins with synaptic vesicles. Both experimental procedures revealed the specific occurrence of multiple small GTP-binding proteins with this organelle. Moreover, direct amino acid sequence analysis assigned at least three different small GTP-binding proteins, ora3, o-ral and o-rab3, to the vesicular compartment. Furthermore, the data reflect the relative abundance of these three proteins on the vesicle membrane, thereby demonstrating the predominant occurrence of o-rab3, the only exclusively synaptic vesicle specific small GTP-binding protein.

Original languageEnglish (US)
Pages (from-to)53-56
Number of pages4
JournalFEBS Letters
Volume317
Issue number1-2
DOIs
StatePublished - Feb 8 1993
Externally publishedYes

Fingerprint

Synaptic Vesicles
GTP-Binding Proteins
Cholinergic Agents
Electric Organ
Torpedo
Protein Sequence Analysis
Guanosine Triphosphate
Organelles
Molecular Weight
Molecular weight
Membranes
Amino Acids
Proteins

Keywords

  • Cholinergic synaptic vesicle
  • Electric ray electric organ
  • GTP-binding protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Association of three small GTP-binding proteins with cholinergic synaptic vesicles. / Volknandt, Walter; Pevsne, Jonathan; Elferink, Lisa; Scheller, Richard H.

In: FEBS Letters, Vol. 317, No. 1-2, 08.02.1993, p. 53-56.

Research output: Contribution to journalArticle

Volknandt, Walter ; Pevsne, Jonathan ; Elferink, Lisa ; Scheller, Richard H. / Association of three small GTP-binding proteins with cholinergic synaptic vesicles. In: FEBS Letters. 1993 ; Vol. 317, No. 1-2. pp. 53-56.
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