Abstract
Several small (low molecular weight) GTP-binding proteins are associated with cholinergic synaptic vesicles derived from the electric organ of electric ray. Using GTP overlay techniques and direct micro sequencing we analyzed the association of small GTP-binding proteins with synaptic vesicles. Both experimental procedures revealed the specific occurrence of multiple small GTP-binding proteins with this organelle. Moreover, direct amino acid sequence analysis assigned at least three different small GTP-binding proteins, ora3, o-ral and o-rab3, to the vesicular compartment. Furthermore, the data reflect the relative abundance of these three proteins on the vesicle membrane, thereby demonstrating the predominant occurrence of o-rab3, the only exclusively synaptic vesicle specific small GTP-binding protein.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 53-56 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 317 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Feb 8 1993 |
| Externally published | Yes |
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Keywords
- Cholinergic synaptic vesicle
- Electric ray electric organ
- GTP-binding protein
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Association of three small GTP-binding proteins with cholinergic synaptic vesicles. / Volknandt, Walter; Pevsne, Jonathan; Elferink, Lisa; Scheller, Richard H.
In: FEBS Letters, Vol. 317, No. 1-2, 08.02.1993, p. 53-56.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of three small GTP-binding proteins with cholinergic synaptic vesicles
AU - Volknandt, Walter
AU - Pevsne, Jonathan
AU - Elferink, Lisa
AU - Scheller, Richard H.
PY - 1993/2/8
Y1 - 1993/2/8
N2 - Several small (low molecular weight) GTP-binding proteins are associated with cholinergic synaptic vesicles derived from the electric organ of electric ray. Using GTP overlay techniques and direct micro sequencing we analyzed the association of small GTP-binding proteins with synaptic vesicles. Both experimental procedures revealed the specific occurrence of multiple small GTP-binding proteins with this organelle. Moreover, direct amino acid sequence analysis assigned at least three different small GTP-binding proteins, ora3, o-ral and o-rab3, to the vesicular compartment. Furthermore, the data reflect the relative abundance of these three proteins on the vesicle membrane, thereby demonstrating the predominant occurrence of o-rab3, the only exclusively synaptic vesicle specific small GTP-binding protein.
AB - Several small (low molecular weight) GTP-binding proteins are associated with cholinergic synaptic vesicles derived from the electric organ of electric ray. Using GTP overlay techniques and direct micro sequencing we analyzed the association of small GTP-binding proteins with synaptic vesicles. Both experimental procedures revealed the specific occurrence of multiple small GTP-binding proteins with this organelle. Moreover, direct amino acid sequence analysis assigned at least three different small GTP-binding proteins, ora3, o-ral and o-rab3, to the vesicular compartment. Furthermore, the data reflect the relative abundance of these three proteins on the vesicle membrane, thereby demonstrating the predominant occurrence of o-rab3, the only exclusively synaptic vesicle specific small GTP-binding protein.
KW - Cholinergic synaptic vesicle
KW - Electric ray electric organ
KW - GTP-binding protein
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U2 - 10.1016/0014-5793(93)81490-Q
DO - 10.1016/0014-5793(93)81490-Q
M3 - Article
C2 - 8428634
AN - SCOPUS:0027533461
VL - 317
SP - 53
EP - 56
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -