Association study of the CTH 1364 G>T polymorphism with coronary artery disease in the Greek population

Efstathia Giannakopoulou, Fotios Konstantinou, Georgia Ragia, Zisis Gerontitis, Anna Tavridou, Andreas Papapetropoulos, Dimitrios Mikroulis, Vangelis G. Manolopoulos

Research output: Contribution to journalArticle

Abstract

Cystathionine γ-lyase enzyme, which is encoded by the CTH gene, is responsible for hydrogen sulfide (H 2 S) production in the endothelium. The CTH 1364 G>T polymorphism may alter the CTH expression and H 2 S bioavailability, thus leading to atherosclerosis and coronary artery disease (CAD). We examined the potential association of the CTH 1364 G>T polymorphism with CAD. The CTH 1364 G>T polymorphism was determined in 178 coronary artery bypass grafting (CABG) patients and 156 non-atherosclerotic controls of Greek Caucasian origin using the PCR-RFLP method. No significant difference in the frequency of the CTH 1364 G>T genotypes (p = 0.281) and alleles (p = 0.265) was found between the CABG patients and controls. After conducting stratification according to sex, analysis showed a numerical difference in the CTH 1364 TT genotype frequency in female participants that did not reach statistical significance (16.3% and 8.5% in the CABG and controls, respectively, p = 0.26). The frequency of the CTH 1364 TT genotype between the male CABG patients and controls did not differ (p = 0.507). The CTH 1364 G>T polymorphism was not associated with CAD in the studied population. However, interestingly, a higher - if not significantly so - CTH 1364 TT genotype frequency was present in female CABG patients compared with female controls. Larger studies are necessary to conclude on the potential overall or gender-driven association between CTH 1364 G>T gene polymorphism and CAD.

Original languageEnglish (US)
Article number20180033
JournalDrug Metabolism and Personalized Therapy
Volume34
Issue number1
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

Coronary Artery Bypass
Coronary Artery Disease
Genotype
Population
Cystathionine
Hydrogen Sulfide
Lyases
Restriction Fragment Length Polymorphisms
Biological Availability
Genes
Endothelium
Atherosclerosis
Alleles
Polymerase Chain Reaction
Enzymes

Keywords

  • coronary artery disease
  • CTH genetic variation
  • cystathionine γ-lyase
  • hydrogen sulfide
  • polymorphism

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

Cite this

Association study of the CTH 1364 G>T polymorphism with coronary artery disease in the Greek population. / Giannakopoulou, Efstathia; Konstantinou, Fotios; Ragia, Georgia; Gerontitis, Zisis; Tavridou, Anna; Papapetropoulos, Andreas; Mikroulis, Dimitrios; Manolopoulos, Vangelis G.

In: Drug Metabolism and Personalized Therapy, Vol. 34, No. 1, 20180033, 01.01.2019.

Research output: Contribution to journalArticle

Giannakopoulou, E, Konstantinou, F, Ragia, G, Gerontitis, Z, Tavridou, A, Papapetropoulos, A, Mikroulis, D & Manolopoulos, VG 2019, 'Association study of the CTH 1364 G>T polymorphism with coronary artery disease in the Greek population' Drug Metabolism and Personalized Therapy, vol. 34, no. 1, 20180033. https://doi.org/10.1515/dmpt-2018-0033
Giannakopoulou, Efstathia ; Konstantinou, Fotios ; Ragia, Georgia ; Gerontitis, Zisis ; Tavridou, Anna ; Papapetropoulos, Andreas ; Mikroulis, Dimitrios ; Manolopoulos, Vangelis G. / Association study of the CTH 1364 G>T polymorphism with coronary artery disease in the Greek population. In: Drug Metabolism and Personalized Therapy. 2019 ; Vol. 34, No. 1.
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