TY - JOUR
T1 - Asthma, allergy, and airway hyperresponsiveness are not linked to the β2-adrenoceptor gene
AU - Emala, Charles W.
AU - McQuitty, Christopher K.
AU - Eleff, Scott M.
AU - Hopkins-Price, Patricia
AU - Lawyer, Carl
AU - Hoh, Josephine
AU - Ott, Jurg
AU - Levine, Michael A.
AU - Hirshman, Carol A.
N1 - Funding Information:
Supported by National Institutes of Health grants HL-45974, HL-02693, and HG-00008.
PY - 2002
Y1 - 2002
N2 - Study objectives: To exclude genetic linkage between the β2-adrenoceptor gene and asthma, allergy, and methacholine airway hyperresponsiveness. Design: The current study used six distinct intragene markers within the β2-adrenoceptor gene, and evaluated genetic linkage between the β2-adrenoceptor and asthma, allergy, or methacholine airway hyperresponsiveness in eight multiplex families. Patients: Forty-nine members of eight multiplex families with a high incidence of asthma. Interventions: Phenotypes were characterized by history, physical examination, skin testing, pulmonary function tests, and methacholine inhalational challenge. Genetic loci were identified using restriction fragment length polymorphisms, denaturing gradient gel electrophoresis, and restriction enzyme digest of polymerase chain reaction-amplified fragments of the β2-adrenoceptor gene. Measurements and results: Nonparametric analysis using computer analysis software found no evidence for linkage between these markers within the β2-adrenoceptor gene and asthma. Parametric exclusion analysis using a dominant inheritance model resulted in large negative lod scores (- 6.74, - 19.44, and - 49.9, respectively) for tight linkage between asthma, allergy, or methacholine airway hyperresponsiveness and these polymorphic markers. Conclusions: These results indicate that asthma, allergy, and methacholine airway hyperresponsiveness are not linked to a dominant β2-adrenoceptor gene with strong effect in these eight families with an inherited pattern of asthma.
AB - Study objectives: To exclude genetic linkage between the β2-adrenoceptor gene and asthma, allergy, and methacholine airway hyperresponsiveness. Design: The current study used six distinct intragene markers within the β2-adrenoceptor gene, and evaluated genetic linkage between the β2-adrenoceptor and asthma, allergy, or methacholine airway hyperresponsiveness in eight multiplex families. Patients: Forty-nine members of eight multiplex families with a high incidence of asthma. Interventions: Phenotypes were characterized by history, physical examination, skin testing, pulmonary function tests, and methacholine inhalational challenge. Genetic loci were identified using restriction fragment length polymorphisms, denaturing gradient gel electrophoresis, and restriction enzyme digest of polymerase chain reaction-amplified fragments of the β2-adrenoceptor gene. Measurements and results: Nonparametric analysis using computer analysis software found no evidence for linkage between these markers within the β2-adrenoceptor gene and asthma. Parametric exclusion analysis using a dominant inheritance model resulted in large negative lod scores (- 6.74, - 19.44, and - 49.9, respectively) for tight linkage between asthma, allergy, or methacholine airway hyperresponsiveness and these polymorphic markers. Conclusions: These results indicate that asthma, allergy, and methacholine airway hyperresponsiveness are not linked to a dominant β2-adrenoceptor gene with strong effect in these eight families with an inherited pattern of asthma.
KW - Airway hyperresponsiveness
KW - Allergy
KW - Asthma
KW - Methacholine
KW - Polymorphism
KW - βadrenoceptor gene
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U2 - 10.1378/chest.121.3.722
DO - 10.1378/chest.121.3.722
M3 - Article
C2 - 11888952
AN - SCOPUS:0036205399
SN - 0012-3692
VL - 121
SP - 722
EP - 731
JO - Chest
JF - Chest
IS - 3
ER -