A3 adenosine receptor signaling contributes to airway inflammation and mucus production in adenosine deaminase-deficient mice

Hays W J Young, Jose G. Molina, Dawn Dimina, Hongyan Zhong, Marlene Jacobson, Lee Nien L Chan, Teh Sheng Chan, James J. Lee, Michael R. Blackburn

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111 Scopus citations

Abstract

Adenosine signaling has been implicated in chronic lung diseases such as asthma and chronic obstructive pulmonary disease; however, the specific roles of the various adenosine receptors in processes central to these disorders are not well understood. In this study, we have investigated the role(s) of the A 3 adenosine receptor in adenosine-dependent pulmonary inflammation observed in adenosine deaminase (ADA)-deficient mice. The A3 receptor (A3R) was found to be expressed in eosinophils and mucusproducing cells in the airways of ADA-deficient mice. Treatment of ADA-deficient mice with MRS 1523, a selective A3R antagonist, prevented airway eosinophilia and mucus production. Similar findings were seen in the lungs of ADA/A 3 double knockout mice. Although eosinophils were decreased in the airways of ADA-deficient mice following antagonism or removal of the A 3R, elevations in circulating and lung interstitial eosinophils persisted, suggesting signaling through the A3R is needed for the migration of eosinophils into the airways. These findings identify an important role for the A3R in regulating lung eosinophilia and mucus production in an environment of elevated adenosine.

Original languageEnglish (US)
Pages (from-to)1380-1389
Number of pages10
JournalJournal of Immunology
Volume173
Issue number2
StatePublished - Jul 15 2004

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ASJC Scopus subject areas

  • Immunology

Cite this

Young, H. W. J., Molina, J. G., Dimina, D., Zhong, H., Jacobson, M., Chan, L. N. L., Chan, T. S., Lee, J. J., & Blackburn, M. R. (2004). A3 adenosine receptor signaling contributes to airway inflammation and mucus production in adenosine deaminase-deficient mice. Journal of Immunology, 173(2), 1380-1389.