Atomic structure of cucumber necrosis virus and the role of the capsid in vector transmission

Ming Li, Kishore Kakani, Umesh Katpally, Sharnice Johnson, D'Ann Rochon, Thomas Smith

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cucumber necrosis virus (CNV) is a member of the genus Tombusvirus and has a monopartite positive-sense RNA genome packaged in a T=3 icosahedral particle. CNV is transmitted in nature via zoospores of the fungus Olpidium bornovanus. CNV undergoes a conformational change upon binding to the zoospore that is required for transmission, and specific polysaccharides on the zoospore surface have been implicated in binding. To better understand this transmission process, we have determined the atomic structure of CNV. As expected, being a member of the Tombusvirus genus, the core structure of CNV is highly similar to that of Tomato bushy stunt virus (TBSV), with major differences lying on the exposed loops. Also, as was seen with TBSV, CNV appears to have a calcium binding site between the subunits around the quasi-3-fold axes. However, unlike TBSV, there appears to be a novel zinc binding site within the β annulus formed by the N termini of the three C subunits at the icosahedral 3-fold axes. Two of the mutations causing defective transmission map immediately around this zinc binding site. The other mutations causing defective transmission and particle formation are mapped onto the CNV structure, and it is likely that a number of the mutations affect zoospore transmission by affecting conformational transitions rather than directly affecting receptor binding.

Original languageEnglish (US)
Pages (from-to)12166-12175
Number of pages10
JournalJournal of Virology
Volume87
Issue number22
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Cucumber necrosis virus
Cucumis sativus
capsid
Tombusvirus
Capsid
Necrosis
Tomato bushy stunt virus
Viruses
zoospores
binding sites
Binding Sites
mutation
Mutation
Zinc
Olpidium
zinc
viral morphology
Viral Structures
Polysaccharides
Fungi

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Medicine(all)

Cite this

Atomic structure of cucumber necrosis virus and the role of the capsid in vector transmission. / Li, Ming; Kakani, Kishore; Katpally, Umesh; Johnson, Sharnice; Rochon, D'Ann; Smith, Thomas.

In: Journal of Virology, Vol. 87, No. 22, 2013, p. 12166-12175.

Research output: Contribution to journalArticle

Li, Ming ; Kakani, Kishore ; Katpally, Umesh ; Johnson, Sharnice ; Rochon, D'Ann ; Smith, Thomas. / Atomic structure of cucumber necrosis virus and the role of the capsid in vector transmission. In: Journal of Virology. 2013 ; Vol. 87, No. 22. pp. 12166-12175.
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