Atorvastatin attenuates neuropathic pain in rat neuropathy model by down-regulating oxidative damage at peripheral, spinal and supraspinal levels

Nitya N. Pathak, Venkanna Balaganur, Madhu C. Lingaraju, Vinay Kant, Najeeb Latief, Amar S. More, Dhirendra Kumar, Dinesh Kumar, Surendra K. Tandan

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Atorvastatin is an HMG-CoA reductase inhibitor used in the treatment of hypercholesterolemia and prevention of coronary heart disease. Oxidative stress is considered to be one of the main causes of neuropathic pain after nerve injury. This study aimed to investigate the effect of atorvastatin on oxidative stress and hyperalgesia in chronic constriction injury (CCI) model of neuropathic pain. Pain behaviour in rats was evaluated before and after atorvastatin administration using mechanical and heat hyperalgesia. The markers for oxidative stress in sciatic nerve, spinal cord and pre-frontal cortex (PFC) area of brain were biochemically detected in vehicle and atorvastatin-treated neuropathic CCI rats. Atorvastatin attenuated hyperalgesia. We found a significant increase in malondialdehyde (MDA), nitric oxide (NO), superoxide anion (O2-) and protein carbonyl along with a reduction in catalase (CAT), reduced glutathione (GSH), total thiol (SH) and glutathione-S-transferase (GST) and; increase in superoxide dismutase (SOD) levels in the sciatic nerve, spinal cord and PFC of the CCI-induced neuropathic rats. Reduced levels of enzymatic and non enzymatic antioxidants were restored by atorvastatin. The levels of MDA, O2-, and protein carbonyl in these tissues were significantly reduced in the atorvastatin-treated CCI rats compared to the untreated CCI rats. Our study demonstrated that atorvastatin attenuates neuropathic pain through inhibition of oxidative stress in sciatic nerve, spinal cord and brain suggesting antioxidants as potential drugs in neuropathic pain management. This study provides a new application of atorvastatin in treatment of neuropathic pain.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalNeurochemistry International
Volume68
Issue number1
DOIs
StatePublished - Mar 2014
Externally publishedYes

Fingerprint

Neuralgia
Constriction
Hyperalgesia
Wounds and Injuries
Oxidative Stress
Sciatic Nerve
Spinal Cord
Frontal Lobe
Malondialdehyde
Antioxidants
Atorvastatin Calcium
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Brain
Pain Management
Hypercholesterolemia
Glutathione Transferase
Sulfhydryl Compounds
Superoxides
Catalase
Superoxide Dismutase

Keywords

  • Atorvastatin
  • Chronic constriction injury
  • Hyperalgesia
  • Neuropathic pain
  • Oxidative stress

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Medicine(all)

Cite this

Atorvastatin attenuates neuropathic pain in rat neuropathy model by down-regulating oxidative damage at peripheral, spinal and supraspinal levels. / Pathak, Nitya N.; Balaganur, Venkanna; Lingaraju, Madhu C.; Kant, Vinay; Latief, Najeeb; More, Amar S.; Kumar, Dhirendra; Kumar, Dinesh; Tandan, Surendra K.

In: Neurochemistry International, Vol. 68, No. 1, 03.2014, p. 1-9.

Research output: Contribution to journalArticle

Pathak, Nitya N. ; Balaganur, Venkanna ; Lingaraju, Madhu C. ; Kant, Vinay ; Latief, Najeeb ; More, Amar S. ; Kumar, Dhirendra ; Kumar, Dinesh ; Tandan, Surendra K. / Atorvastatin attenuates neuropathic pain in rat neuropathy model by down-regulating oxidative damage at peripheral, spinal and supraspinal levels. In: Neurochemistry International. 2014 ; Vol. 68, No. 1. pp. 1-9.
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