ATP from glycolysis is required for normal sodium homeostasis in resting fast-twitch rodent skeletal muscle

K. E N Okamoto, Weiyang Wang, J. A N Rounds, Elizabeth A. Chambers, Danny O. Jacobs

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Myocellular sodium homeostasis is commonly disrupted during critical illness for unknown reasons. Recent data suggest that changes in intracellular sodium content and the amount of ATP provided by glycolysis are closely related. The role of glycolysis and oxidative phosphorylation in providing fuel to the Na+-K+ pump was investigated in resting rat extensor digitorum longus muscles incubated at 30°C for 1 h. Oxidative inhibition with carbonyl cyanide m-chlorophenylhydrazone, known as CCCP (0.2 μM), or by hypooxygenation did not alter myocellular sodium or potassium content ([Na+]i, [K+]i, respectively), whereas treatment with iodoacetic acid (0.3 mM), which effectively blocked glycolysis, dramatically increased [Na+]i and the [Na+]i/[K+]i ratio. Experiments using ouabain and measurements of myocellular high-energy phosphates indicate that Na+-K+-ATPase activity is only impaired when glycolysis is inhibited. The data suggest that normal glycolysis is required to regulate intracellular sodium in fast-twitch skeletal muscles, because it is the predominant source of the fuel for the Na+-K+ -ATPase.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume281
Issue number3 44-3
StatePublished - 2001
Externally publishedYes

Fingerprint

Glycolysis
Muscle
Rodentia
Skeletal Muscle
Homeostasis
Adenosine Triphosphate
Sodium
Adenosine Triphosphatases
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Iodoacetic Acid
Oxidative Phosphorylation
Ouabain
Critical Illness
Rats
Potassium
Phosphates
Pumps
Muscles
Experiments
sodium-translocating ATPase

Keywords

  • High-energy phosphate
  • Metabolic inhibition
  • Oxidative phosphorylation
  • Sodium-potassium-adenosine triphosphatase

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

ATP from glycolysis is required for normal sodium homeostasis in resting fast-twitch rodent skeletal muscle. / Okamoto, K. E N; Wang, Weiyang; Rounds, J. A N; Chambers, Elizabeth A.; Jacobs, Danny O.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 281, No. 3 44-3, 2001.

Research output: Contribution to journalArticle

Okamoto, K. E N ; Wang, Weiyang ; Rounds, J. A N ; Chambers, Elizabeth A. ; Jacobs, Danny O. / ATP from glycolysis is required for normal sodium homeostasis in resting fast-twitch rodent skeletal muscle. In: American Journal of Physiology - Endocrinology and Metabolism. 2001 ; Vol. 281, No. 3 44-3.
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