Attachment of LcrV from Yersinia pestis at dual binding sites to human TLR-2 and human IFN-γ receptor

Vyacheslav M. Abramov, Valentin S. Khlebnikov, Anatoly M. Vasiliev, Igor V. Kosarev, Raisa N. Vasilenko, Nataly L. Kulikova, Anna V. Khodyakova, Valentin I. Evstigneev, Vladimir N. Uversky, Vladimir Motin, Georgy B. Smirnov, Robert R. Brubaker

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The virulence antigen (V-antigen, LcrV) of Yersinia pestis, the causative agent of bubonic plague, is an established protective antigen known to regulate, target, and mediate type III translocation of cytotoxic yersiniae outer proteins termed Yops; LcrV also prompts TLR2-dependent upregulation of anti-inflammatory IL-10. In this study, we determined the parameters of specific interaction of LcrV with TLR2 expressed on human transfected HEK293 cells (TLR2+/CD14-), VTEC2.HS cells (TLR2+/CD14 -), primary monocytes (TLR2+/CD14+), and THP-1 cells (TLR2+/CD14+). The IRRL314-317 motif of the extracellular domain of human and mouse TLR2 accounted for high-affinity binding of LcrV. The CD14 co-receptor did not influence this interaction. LcrV did not bind to human U937 (TLR2-/CD14-) and alveolar macrophages (TLR2-/CD14+) in the absence of receptor-bound human IFN-γ or a synthetic C-terminal fragment (hIFN-γ 132-143). The latter, but not mouse IFN-γ (or synthetic control peptides), shared a GRRA138-141 site necessary for high-affinity specific binding. LcrV of V. pestis shares the N-terminal LEEL32-35 binding site of Yersinia enterocolitica and also has an exposed internal DEEI203-206 binding site. Comparison of binding constants and consideration of steric restrictions indicate that binding is not cooperative and only the internal site binds LcrV to target cells. Both the LEEL 32-35 and DEEI203-206 binding sites are removed by five amino acids from DKN residues associated with biological activity of bound LcrV. LcrV of Y. pestis promoted both TLR2/CD14-dependent and TLR2/CD14-independent amplification of IL-10 and concomitant downregulation of TNF-α in human target cells. The ability of LcrV to utilize human IFN-γ (a major inflammatory effector of innate immunity) to minimize inflammation is insidious and may account in part for the severe symptoms of plague in man.

Original languageEnglish (US)
Pages (from-to)2222-2231
Number of pages10
JournalJournal of Proteome Research
Volume6
Issue number6
DOIs
StatePublished - Jun 2007

Fingerprint

Yersinia pestis
Binding Sites
Antigens
Interleukin-10
Plague
Bioactivity
Amplification
Anti-Inflammatory Agents
Amino Acids
Yersinia
Yersinia enterocolitica
Peptides
HEK293 Cells
Alveolar Macrophages
Innate Immunity
Virulence
Monocytes
Proteins
Up-Regulation
Down-Regulation

Keywords

  • Interferon-γ
  • Plague
  • Toll-like receptor TLR2
  • Virulence antigen LcrV
  • Yersinia pestis

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Abramov, V. M., Khlebnikov, V. S., Vasiliev, A. M., Kosarev, I. V., Vasilenko, R. N., Kulikova, N. L., ... Brubaker, R. R. (2007). Attachment of LcrV from Yersinia pestis at dual binding sites to human TLR-2 and human IFN-γ receptor. Journal of Proteome Research, 6(6), 2222-2231. https://doi.org/10.1021/pr070036r

Attachment of LcrV from Yersinia pestis at dual binding sites to human TLR-2 and human IFN-γ receptor. / Abramov, Vyacheslav M.; Khlebnikov, Valentin S.; Vasiliev, Anatoly M.; Kosarev, Igor V.; Vasilenko, Raisa N.; Kulikova, Nataly L.; Khodyakova, Anna V.; Evstigneev, Valentin I.; Uversky, Vladimir N.; Motin, Vladimir; Smirnov, Georgy B.; Brubaker, Robert R.

In: Journal of Proteome Research, Vol. 6, No. 6, 06.2007, p. 2222-2231.

Research output: Contribution to journalArticle

Abramov, VM, Khlebnikov, VS, Vasiliev, AM, Kosarev, IV, Vasilenko, RN, Kulikova, NL, Khodyakova, AV, Evstigneev, VI, Uversky, VN, Motin, V, Smirnov, GB & Brubaker, RR 2007, 'Attachment of LcrV from Yersinia pestis at dual binding sites to human TLR-2 and human IFN-γ receptor', Journal of Proteome Research, vol. 6, no. 6, pp. 2222-2231. https://doi.org/10.1021/pr070036r
Abramov VM, Khlebnikov VS, Vasiliev AM, Kosarev IV, Vasilenko RN, Kulikova NL et al. Attachment of LcrV from Yersinia pestis at dual binding sites to human TLR-2 and human IFN-γ receptor. Journal of Proteome Research. 2007 Jun;6(6):2222-2231. https://doi.org/10.1021/pr070036r
Abramov, Vyacheslav M. ; Khlebnikov, Valentin S. ; Vasiliev, Anatoly M. ; Kosarev, Igor V. ; Vasilenko, Raisa N. ; Kulikova, Nataly L. ; Khodyakova, Anna V. ; Evstigneev, Valentin I. ; Uversky, Vladimir N. ; Motin, Vladimir ; Smirnov, Georgy B. ; Brubaker, Robert R. / Attachment of LcrV from Yersinia pestis at dual binding sites to human TLR-2 and human IFN-γ receptor. In: Journal of Proteome Research. 2007 ; Vol. 6, No. 6. pp. 2222-2231.
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AU - Abramov, Vyacheslav M.

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AU - Vasiliev, Anatoly M.

AU - Kosarev, Igor V.

AU - Vasilenko, Raisa N.

AU - Kulikova, Nataly L.

AU - Khodyakova, Anna V.

AU - Evstigneev, Valentin I.

AU - Uversky, Vladimir N.

AU - Motin, Vladimir

AU - Smirnov, Georgy B.

AU - Brubaker, Robert R.

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N2 - The virulence antigen (V-antigen, LcrV) of Yersinia pestis, the causative agent of bubonic plague, is an established protective antigen known to regulate, target, and mediate type III translocation of cytotoxic yersiniae outer proteins termed Yops; LcrV also prompts TLR2-dependent upregulation of anti-inflammatory IL-10. In this study, we determined the parameters of specific interaction of LcrV with TLR2 expressed on human transfected HEK293 cells (TLR2+/CD14-), VTEC2.HS cells (TLR2+/CD14 -), primary monocytes (TLR2+/CD14+), and THP-1 cells (TLR2+/CD14+). The IRRL314-317 motif of the extracellular domain of human and mouse TLR2 accounted for high-affinity binding of LcrV. The CD14 co-receptor did not influence this interaction. LcrV did not bind to human U937 (TLR2-/CD14-) and alveolar macrophages (TLR2-/CD14+) in the absence of receptor-bound human IFN-γ or a synthetic C-terminal fragment (hIFN-γ 132-143). The latter, but not mouse IFN-γ (or synthetic control peptides), shared a GRRA138-141 site necessary for high-affinity specific binding. LcrV of V. pestis shares the N-terminal LEEL32-35 binding site of Yersinia enterocolitica and also has an exposed internal DEEI203-206 binding site. Comparison of binding constants and consideration of steric restrictions indicate that binding is not cooperative and only the internal site binds LcrV to target cells. Both the LEEL 32-35 and DEEI203-206 binding sites are removed by five amino acids from DKN residues associated with biological activity of bound LcrV. LcrV of Y. pestis promoted both TLR2/CD14-dependent and TLR2/CD14-independent amplification of IL-10 and concomitant downregulation of TNF-α in human target cells. The ability of LcrV to utilize human IFN-γ (a major inflammatory effector of innate immunity) to minimize inflammation is insidious and may account in part for the severe symptoms of plague in man.

AB - The virulence antigen (V-antigen, LcrV) of Yersinia pestis, the causative agent of bubonic plague, is an established protective antigen known to regulate, target, and mediate type III translocation of cytotoxic yersiniae outer proteins termed Yops; LcrV also prompts TLR2-dependent upregulation of anti-inflammatory IL-10. In this study, we determined the parameters of specific interaction of LcrV with TLR2 expressed on human transfected HEK293 cells (TLR2+/CD14-), VTEC2.HS cells (TLR2+/CD14 -), primary monocytes (TLR2+/CD14+), and THP-1 cells (TLR2+/CD14+). The IRRL314-317 motif of the extracellular domain of human and mouse TLR2 accounted for high-affinity binding of LcrV. The CD14 co-receptor did not influence this interaction. LcrV did not bind to human U937 (TLR2-/CD14-) and alveolar macrophages (TLR2-/CD14+) in the absence of receptor-bound human IFN-γ or a synthetic C-terminal fragment (hIFN-γ 132-143). The latter, but not mouse IFN-γ (or synthetic control peptides), shared a GRRA138-141 site necessary for high-affinity specific binding. LcrV of V. pestis shares the N-terminal LEEL32-35 binding site of Yersinia enterocolitica and also has an exposed internal DEEI203-206 binding site. Comparison of binding constants and consideration of steric restrictions indicate that binding is not cooperative and only the internal site binds LcrV to target cells. Both the LEEL 32-35 and DEEI203-206 binding sites are removed by five amino acids from DKN residues associated with biological activity of bound LcrV. LcrV of Y. pestis promoted both TLR2/CD14-dependent and TLR2/CD14-independent amplification of IL-10 and concomitant downregulation of TNF-α in human target cells. The ability of LcrV to utilize human IFN-γ (a major inflammatory effector of innate immunity) to minimize inflammation is insidious and may account in part for the severe symptoms of plague in man.

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