Attenuation of Venezuelan equine encephalitis virus strain TC-83 is encoded by the 5′-noncoding region and the E2 envelope glycoprotein

Richard M. Kinney, Gwong Jen Chang, Kiyotaka R. Tsuchiya, Judith M. Sneider, John T. Roehrig, Tonja M. Woodward, Dennis W. Trent

Research output: Contribution to journalArticle

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Abstract

The virulent Trinidad donkey (TRD) strain of Venezuelan equine encephalitis (VEE) virus and its live attenuated vaccine derivative, TC-83 virus, have different neurovirulence characteristics. A full-length cDNA clone of the TC-83 virus genome was constructed behind the bacteriophage T7 promoter in the polylinker of plasmid pUC18. To identify the genomic determinants of TC-83 virus attenuation, TRD virus-specific sequences were inserted into the TC-83 virus clone by in vitro mutagenesis or recombination. Antigenic analysis of recombinant viruses with VEE E2- and El-specific monoclonal antibodies gave predicted antigenic reactivities. Mouse challenge experiments indicated that genetic markers responsible for the attenuated phenotype of TC-83 virus are composed of genome nucleotide position 3 in the 5′-noncoding region and the E2 envelope glycoprotein. TC-83 virus amino acid position E2-120 appeared to be the major structural determinant of attenuation. Insertion of the TRD virus-specific 5′-noncoding region, by itself, into the TC-83 virus full-length clone did not alter the attenuated phenotype of the virus. However, the TRD virus-specific 5′-noncoding region enhanced the virulence potential of downstream TRD virus amino acid sequences.

Original languageEnglish (US)
Pages (from-to)1269-1277
Number of pages9
JournalJournal of Virology
Volume67
Issue number3
StatePublished - Mar 1993

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Venezuelan Equine Encephalitis Viruses
Venezuelan equine encephalitis virus
glycoproteins
Glycoproteins
Viruses
viruses
Trinidad and Tobago
Equidae
asses
Clone Cells
live vaccines
clones
Genome
Bacteriophage T7
Phenotype
Attenuated Vaccines
phenotype
genome
Genetic Markers
encephalitis

ASJC Scopus subject areas

  • Immunology

Cite this

Kinney, R. M., Chang, G. J., Tsuchiya, K. R., Sneider, J. M., Roehrig, J. T., Woodward, T. M., & Trent, D. W. (1993). Attenuation of Venezuelan equine encephalitis virus strain TC-83 is encoded by the 5′-noncoding region and the E2 envelope glycoprotein. Journal of Virology, 67(3), 1269-1277.

Attenuation of Venezuelan equine encephalitis virus strain TC-83 is encoded by the 5′-noncoding region and the E2 envelope glycoprotein. / Kinney, Richard M.; Chang, Gwong Jen; Tsuchiya, Kiyotaka R.; Sneider, Judith M.; Roehrig, John T.; Woodward, Tonja M.; Trent, Dennis W.

In: Journal of Virology, Vol. 67, No. 3, 03.1993, p. 1269-1277.

Research output: Contribution to journalArticle

Kinney, RM, Chang, GJ, Tsuchiya, KR, Sneider, JM, Roehrig, JT, Woodward, TM & Trent, DW 1993, 'Attenuation of Venezuelan equine encephalitis virus strain TC-83 is encoded by the 5′-noncoding region and the E2 envelope glycoprotein', Journal of Virology, vol. 67, no. 3, pp. 1269-1277.
Kinney RM, Chang GJ, Tsuchiya KR, Sneider JM, Roehrig JT, Woodward TM et al. Attenuation of Venezuelan equine encephalitis virus strain TC-83 is encoded by the 5′-noncoding region and the E2 envelope glycoprotein. Journal of Virology. 1993 Mar;67(3):1269-1277.
Kinney, Richard M. ; Chang, Gwong Jen ; Tsuchiya, Kiyotaka R. ; Sneider, Judith M. ; Roehrig, John T. ; Woodward, Tonja M. ; Trent, Dennis W. / Attenuation of Venezuelan equine encephalitis virus strain TC-83 is encoded by the 5′-noncoding region and the E2 envelope glycoprotein. In: Journal of Virology. 1993 ; Vol. 67, No. 3. pp. 1269-1277.
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