Attenuation phenotype of a cell culture-adapted variant of hepatitis A virus (HM175/p16) in Susceptible new world owl monkeys

Kerry L. Taylor, Paula C. Murphy, Ludmila V S Asher, James W. LeDuc, James LeDuc

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13 Scopus citations

Abstract

The virulence of a clonally isolated, cell culture-adapted hepatitis A virus (HM175/p16) was assessed in 4 seronegative owl monkeys inoculated intravenously with 2.8 X 104 radioimmunofocus-forming units of virus. The virus was highly attenuated, even though its complete nucleotide sequence contains only 19 mutations from the wild-type genome. Only 3 monkeys developed antibodies to hepatitis A virus (only 2 within 96 days of virus inoculation). One monkey had viremia and significantly elevated serum aminotransferase levels. In this animal, maximum vire-mia and fecal shedding of virus occurred 30-33 days after inoculation. In contrast, in earlier studies of a related cell culture-adapted but still hepatovirulent virus (HM 175/S18), viremia was documented in 6 of 6 animals and peak viremia and fecal shedding of virus occurred 18 or 19 days after intravenous inoculation of about one-tenth as much virus.

Original languageEnglish (US)
Pages (from-to)592-601
Number of pages10
JournalJournal of Infectious Diseases
Volume168
Issue number3
StatePublished - Sep 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

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