Attenuation Phenotype of a Cell Culture-Adapted Variant of Hepatitis A Virus (HM175/p16) in Susceptible New World Owl Monkeys

Stanley M. Lemon, Kerry L. Taylor, Paula C. Murphy, Ludmila V.S. Asher, James W. LeDuc, Stanley M. Lemon, Kerry L. Taylor, Paula C. Murphy, Ludmila V.S. Asher, James W. LeDuc, Stanley M. Lemon, Kerry L. Taylor, Paula C. Murphy, Ludmila V.S. Asher, James W. LeDuc, James W. LeDuc

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The virulence of a clonally isolated, cell culture-adapted hepatitis A virus (HM175/p16) was assessed in 4 seronegative owl monkeys inoculated intravenously with 2.8 × 104 radioimmunofocus- forming units of virus. The virus was highly attenuated, even though its complete nucleotide sequence contains only 19 mutations from the wild-type genome. Only 3 monkeys developed antibodies to hepatitis A virus (only 2 within 96 days of virus inoculation). One monkey had viremia and significantly elevated serum aminotransferase levels. In this animal, maximum viremia and fecal shedding of virus occurred 30–33 days after inoculation. In contrast, in earlier studies of a related cell culture-adapted but still hepatovirulent virus (HM175/S18), viremia was documented in 6 of6 animals and peak viremia and fecal shedding ofvirus occurred 18 or 19 days after intravenous inoculation of about one-tenth as much virus.

Original languageEnglish (US)
Pages (from-to)592-601
Number of pages10
JournalJournal of Infectious Diseases
Volume168
Issue number3
DOIs
StatePublished - Sep 1993
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Attenuation Phenotype of a Cell Culture-Adapted Variant of Hepatitis A Virus (HM175/p16) in Susceptible New World Owl Monkeys'. Together they form a unique fingerprint.

  • Cite this

    Lemon, S. M., Taylor, K. L., Murphy, P. C., Asher, L. V. S., LeDuc, J. W., Lemon, S. M., Taylor, K. L., Murphy, P. C., Asher, L. V. S., LeDuc, J. W., Lemon, S. M., Taylor, K. L., Murphy, P. C., Asher, L. V. S., LeDuc, J. W., & LeDuc, J. W. (1993). Attenuation Phenotype of a Cell Culture-Adapted Variant of Hepatitis A Virus (HM175/p16) in Susceptible New World Owl Monkeys. Journal of Infectious Diseases, 168(3), 592-601. https://doi.org/10.1093/infdis/168.3.592