Auricular vagal nerve stimulation ameliorates burn-induced gastric dysmotility via sympathetic-COX-2 pathways in rats

H. Li, J. Yin, Z. Zhang, John Winston, Xuan-Zheng Shi, J. D Z Chen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Severe burn injury has been demonstrated to delay gastric emptying. The aim of this study was to investigate effects and cellular mechanisms of auricular electroacupuncture (AEA) at the acupoints innervated by the auricular branch of vagus nerve on burn-induced gastric dysmotility in rats. Methods: Propranolol (β-adrenoceptor antagonist) was injected intraperitoneally after the rats underwent burn injury. All experiments were performed 6 h following burn/sham burn injury. AEA was performed at bilateral auricular acupoints for 45 min. Electrocardiogram was recorded for 30 min. Plasma hormones were measured; cyclooxygenase (COX)-2 expressions in gastric tissue were measured using western blotting and real-time RT-PCR. Key Results: (i) Burn injury delayed gastric emptying (p = 0.006) and AEA increased gastric emptying by 49% (p = 0.045). (ii) Burn injury evoked a significant elevation in plasma noradrenaline, which was suppressed by AEA. (iii) Burn injury significantly increased protein and mRNA expressions of COX-2 in gastric fundus and antrum. AEA suppressed burn-induced increase in protein expressions, but not mRNA expressions of COX-2. Conclusions & Inferences: Burn injury delays gastric emptying by up-regulating COX-2 attributed to sympathetic overactivity. AEA improves burn-induced delay in gastric emptying, possibly mediated via the sympathetic-COX-2 pathway.

Original languageEnglish (US)
Pages (from-to)36-42
Number of pages7
JournalNeurogastroenterology and Motility
Volume28
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Vagus Nerve Stimulation
Electroacupuncture
Cyclooxygenase 2
Burns
Stomach
Gastric Emptying
Wounds and Injuries
Acupuncture Points
Gastric Fundus
Pyloric Antrum
Messenger RNA
Vagus Nerve
Propranolol
Adrenergic Receptors
Real-Time Polymerase Chain Reaction
Norepinephrine
Electrocardiography
Proteins
Western Blotting
Hormones

Keywords

  • Auricular electroacupuncture
  • Autonomic function
  • COX-2
  • Gastric emptying
  • Vagal nerve stimulation

ASJC Scopus subject areas

  • Endocrine and Autonomic Systems
  • Gastroenterology
  • Physiology

Cite this

Auricular vagal nerve stimulation ameliorates burn-induced gastric dysmotility via sympathetic-COX-2 pathways in rats. / Li, H.; Yin, J.; Zhang, Z.; Winston, John; Shi, Xuan-Zheng; Chen, J. D Z.

In: Neurogastroenterology and Motility, Vol. 28, No. 1, 01.01.2016, p. 36-42.

Research output: Contribution to journalArticle

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