Autoinducer AI-2 is involved in regulating a variety of cellular processes in Salmonella Typhimurium

Kamlesh A. Soni, Palmy R. Jesudhasan, Martha Cepeda, Brad Williams, Michael Hume, William Russell, Arul Jayaraman, Suresh D. Pillai

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Salmonella Typhimurium is known to exhibit LuxS/AI-2-mediated cell signaling. We investigated the role of LuxS/AI-2 system on Salmonella Typhimurium protein expression using a proteomics approach based on two-dimensional gel electrophoresis (2DGE)-MALDI-MS. The global protein expression profiles of the wild-type, a luxS mutant, and a luxS mutant strain supplemented with AI-2 were compared. Seven proteins were differentially expressed when comparing the wild-type and luxS mutant strains, whereas 13 proteins were differentially expressed when comparisons were made between luxS mutant strains with and without AI-2 supplementation. The seven proteins that were differentially expressed between the wild-type and the luxS mutant strain were also differentially expressed in the luxS mutant strain supplemented with AI-2. The level of PhoP, a virulence determinant, was higher in the presence of AI-2. Proteins associated with the carbohydrate metabolism (pfkA, gpmI, and talB) and ATP synthesis (Pta gene product) were up-regulated by the presence of AI-2 molecules. These results provide experimental evidence that AI-2 molecules regulate a variety of cellular processes in Salmonella Typhimurium.

Original languageEnglish (US)
Pages (from-to)147-153
Number of pages7
JournalFoodborne Pathogens and Disease
Volume5
Issue number2
DOIs
StatePublished - Apr 1 2008
Externally publishedYes

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Salmonella typhimurium
Salmonella Typhimurium
mutants
Proteins
proteins
protein synthesis
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
two-dimensional gel electrophoresis
Electrophoresis, Gel, Two-Dimensional
Carbohydrate Metabolism
carbohydrate metabolism
Proteomics
proteomics
Virulence
N-octanoylhomoserine lactone
virulence
Adenosine Triphosphate
synthesis
Genes
genes

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Infectious Diseases

Cite this

Autoinducer AI-2 is involved in regulating a variety of cellular processes in Salmonella Typhimurium. / Soni, Kamlesh A.; Jesudhasan, Palmy R.; Cepeda, Martha; Williams, Brad; Hume, Michael; Russell, William; Jayaraman, Arul; Pillai, Suresh D.

In: Foodborne Pathogens and Disease, Vol. 5, No. 2, 01.04.2008, p. 147-153.

Research output: Contribution to journalArticle

Soni, KA, Jesudhasan, PR, Cepeda, M, Williams, B, Hume, M, Russell, W, Jayaraman, A & Pillai, SD 2008, 'Autoinducer AI-2 is involved in regulating a variety of cellular processes in Salmonella Typhimurium', Foodborne Pathogens and Disease, vol. 5, no. 2, pp. 147-153. https://doi.org/10.1089/fpd.2007.0050
Soni, Kamlesh A. ; Jesudhasan, Palmy R. ; Cepeda, Martha ; Williams, Brad ; Hume, Michael ; Russell, William ; Jayaraman, Arul ; Pillai, Suresh D. / Autoinducer AI-2 is involved in regulating a variety of cellular processes in Salmonella Typhimurium. In: Foodborne Pathogens and Disease. 2008 ; Vol. 5, No. 2. pp. 147-153.
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