Autoinducer AI-2 is involved in regulating a variety of cellular processes in Salmonella Typhimurium

Kamlesh A. Soni, Palmy R. Jesudhasan, Martha Cepeda, Brad Williams, Michael Hume, William Russell, Arul Jayaraman, Suresh D. Pillai

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Salmonella Typhimurium is known to exhibit LuxS/AI-2-mediated cell signaling. We investigated the role of LuxS/AI-2 system on Salmonella Typhimurium protein expression using a proteomics approach based on two-dimensional gel electrophoresis (2DGE)-MALDI-MS. The global protein expression profiles of the wild-type, a luxS mutant, and a luxS mutant strain supplemented with AI-2 were compared. Seven proteins were differentially expressed when comparing the wild-type and luxS mutant strains, whereas 13 proteins were differentially expressed when comparisons were made between luxS mutant strains with and without AI-2 supplementation. The seven proteins that were differentially expressed between the wild-type and the luxS mutant strain were also differentially expressed in the luxS mutant strain supplemented with AI-2. The level of PhoP, a virulence determinant, was higher in the presence of AI-2. Proteins associated with the carbohydrate metabolism (pfkA, gpmI, and talB) and ATP synthesis (Pta gene product) were up-regulated by the presence of AI-2 molecules. These results provide experimental evidence that AI-2 molecules regulate a variety of cellular processes in Salmonella Typhimurium.

Original languageEnglish (US)
Pages (from-to)147-153
Number of pages7
JournalFoodborne Pathogens and Disease
Volume5
Issue number2
DOIs
StatePublished - Apr 1 2008
Externally publishedYes

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ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Infectious Diseases

Cite this

Soni, K. A., Jesudhasan, P. R., Cepeda, M., Williams, B., Hume, M., Russell, W., Jayaraman, A., & Pillai, S. D. (2008). Autoinducer AI-2 is involved in regulating a variety of cellular processes in Salmonella Typhimurium. Foodborne Pathogens and Disease, 5(2), 147-153. https://doi.org/10.1089/fpd.2007.0050