Automated capillary electrophoresis applied to therapeutic drug monitoring

M. A. Evenson, J. E. Wiktorowicz

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Chromatographic separations in conjunction with physical chemical detection methods can provide extremely accurate and highly precise measurements of drugs in biological matrices. Immunochemical methods for drug analyses, on the other hand, although usually fully automated, often lack accuracy, may be subject to matrix interference effects, and frequently exhibit nonlinearities that contribute to suboptimal analytical results. Automated capillary electrophoresis (CE) promises the high accuracy and precision of physical methods with no operator intervention once the sample is loaded into the sample cup. A clean-up step is necessary for the analysis of drugs in biological matrices, but that could also be fully automated. Reagent costs per test with CE approach zero, because of the use of extremely small volumes of buffers, standards, and controls. We present data supporting CE as an alternative separation strategy for 28 commonly used therapeutic drugs. These can be resolved in <16 min with an optimized micelle-based buffer system.

Original languageEnglish (US)
Pages (from-to)1847-1852
Number of pages6
JournalClinical chemistry
Volume38
Issue number9
DOIs
StatePublished - 1992
Externally publishedYes

Keywords

  • anticonvulsant drugs
  • barbiturates
  • benzodiazepines
  • drug assay
  • micellar electrochromatography
  • sodium dodecyl sulfate micelles
  • xanthines

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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