Automated capillary electrophoresis applied to therapeutic drug monitoring

M. A. Evenson, J. E. Wiktorowicz

    Research output: Contribution to journalArticle

    48 Scopus citations

    Abstract

    Chromatographic separations in conjunction with physical chemical detection methods can provide extremely accurate and highly precise measurements of drugs in biological matrices. Immunochemical methods for drug analyses, on the other hand, although usually fully automated, often lack accuracy, may be subject to matrix interference effects, and frequently exhibit nonlinearities that contribute to suboptimal analytical results. Automated capillary electrophoresis (CE) promises the high accuracy and precision of physical methods with no operator intervention once the sample is loaded into the sample cup. A clean-up step is necessary for the analysis of drugs in biological matrices, but that could also be fully automated. Reagent costs per test with CE approach zero, because of the use of extremely small volumes of buffers, standards, and controls. We present data supporting CE as an alternative separation strategy for 28 commonly used therapeutic drugs. These can be resolved in <16 min with an optimized micelle-based buffer system.

    Original languageEnglish (US)
    Pages (from-to)1847-1852
    Number of pages6
    JournalClinical chemistry
    Volume38
    Issue number9
    DOIs
    StatePublished - Jan 1 1992

    Keywords

    • anticonvulsant drugs
    • barbiturates
    • benzodiazepines
    • drug assay
    • micellar electrochromatography
    • sodium dodecyl sulfate micelles
    • xanthines

    ASJC Scopus subject areas

    • Clinical Biochemistry
    • Biochemistry, medical

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