Autoradiography, MALDI-MS, and SIMS-MS imaging in pharmaceutical discovery and development

Eric G. Solon, Alain Schweitzer, Markus Stoeckli, Brendan Prideaux

Research output: Contribution to journalReview article

157 Citations (Scopus)

Abstract

Whole-body autoradiography ((WBA) or quantitative WBA (QWBA)), microautoradiography (MARG), matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI), and secondary ion mass spectrometric imaging (SIMS-MSI) are high-resolution, molecular imaging techniques used to study the tissue distribution of radiolabeled and nonlabeled compounds in ex vivo, in situ biological samples. WBA, which is the imaging of the whole-body of lab animals, and/or their organ systems; and MARG, which provides information on the localization of radioactivity in histological preparations and at the cellular level, are used to support drug discovery and development efforts. These studies enable the conduct of human radiolabeled metabolite studies and have provided pharmaceutical scientists with a high resolution and quantitative method of accessing tissue distribution. MALDI-MSI is a mass spectrometric imaging technique capable of label-free and simultaneous determination of the identity and distribution of xenobiotics and their metabolites as well as endogenous substances in biological samples. This makes it an interesting extension to WBA and MARG, eliminating the need for radiochemistry and providing molecular specific information. SIMS-MSI offers a complementary method to MALDI-MSI for the acquisition of images with higher spatial resolution directly from biological specimens. Although traditionally used for the analysis of surface films and polymers, SIMS has been used successfully for the study of biological tissues and cell types, thus enabling the acquisition of images at submicrometer resolution with a minimum of samples preparation.

Original languageEnglish (US)
Pages (from-to)11-26
Number of pages16
JournalAAPS Journal
Volume12
Issue number1
DOIs
StatePublished - Mar 1 2010
Externally publishedYes

Fingerprint

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Autoradiography
Lasers
Tissue Distribution
Pharmaceutical Preparations
Radiochemistry
Whole Body Imaging
Molecular Imaging
Xenobiotics
Drug Discovery
Radioactivity
Polymers
Ions
N-methylsuccinimide

Keywords

  • MALDI-MSI
  • Microautoradiography
  • SIMS-MSI
  • Whole-body autoradiography

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Autoradiography, MALDI-MS, and SIMS-MS imaging in pharmaceutical discovery and development. / Solon, Eric G.; Schweitzer, Alain; Stoeckli, Markus; Prideaux, Brendan.

In: AAPS Journal, Vol. 12, No. 1, 01.03.2010, p. 11-26.

Research output: Contribution to journalReview article

Solon, Eric G. ; Schweitzer, Alain ; Stoeckli, Markus ; Prideaux, Brendan. / Autoradiography, MALDI-MS, and SIMS-MS imaging in pharmaceutical discovery and development. In: AAPS Journal. 2010 ; Vol. 12, No. 1. pp. 11-26.
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