Avian reticuloendotheliosis virus: Identification of the hematopoietic target cell for transformation

Robert B. Lewis, Janela McClure, Bonita Rup, David Niesel, Robert F. Garry, Jacalyn D. Hoelzer, Keyvan Nazerian, Henry R. Bose

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Non-virus-producing hematopoietic cells transformed in vitro by reticuloendotheliosis virus (REV-T) induce lethal "reticuloendotheliosis" when inoculated into histocompatible chickens. This is the first direct demonstration that an in vivo target cell of an avian acute leukemia virus can be transformed in vitro. The tumorigenic, REV-T-transformed non-virus-producing cells fail to express helper-virus-coded proteins. REV-T-transformed tumorigenic cells therefore do not require helper-virus functions. Cells transformed in vivo or in vitro by REV-T have lymphoblastoid morphology and express low levels of terminal-deoxynucleotidyl-transferase activity and bursal-cell determinants. One clone synthesized Ig μ. The preferred target cells for REV-T transformation are therefore immature lymphoid cells that express B-cell determinants. We propose that the unique transforming sequence of REV-T be designated rel (lymphoid).

Original languageEnglish (US)
Pages (from-to)421-431
Number of pages11
JournalCell
Volume25
Issue number2
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

Avian Reticuloendotheliosis Viruses
Viruses
Helper Viruses
DNA Nucleotidylexotransferase
Reticuloendotheliosis virus
Demonstrations
Cells
Chickens
Leukemia
B-Lymphocytes
Clone Cells
Proteins
Lymphocytes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Lewis, R. B., McClure, J., Rup, B., Niesel, D., Garry, R. F., Hoelzer, J. D., ... Bose, H. R. (1981). Avian reticuloendotheliosis virus: Identification of the hematopoietic target cell for transformation. Cell, 25(2), 421-431. https://doi.org/10.1016/0092-8674(81)90060-X

Avian reticuloendotheliosis virus : Identification of the hematopoietic target cell for transformation. / Lewis, Robert B.; McClure, Janela; Rup, Bonita; Niesel, David; Garry, Robert F.; Hoelzer, Jacalyn D.; Nazerian, Keyvan; Bose, Henry R.

In: Cell, Vol. 25, No. 2, 1981, p. 421-431.

Research output: Contribution to journalArticle

Lewis, RB, McClure, J, Rup, B, Niesel, D, Garry, RF, Hoelzer, JD, Nazerian, K & Bose, HR 1981, 'Avian reticuloendotheliosis virus: Identification of the hematopoietic target cell for transformation', Cell, vol. 25, no. 2, pp. 421-431. https://doi.org/10.1016/0092-8674(81)90060-X
Lewis, Robert B. ; McClure, Janela ; Rup, Bonita ; Niesel, David ; Garry, Robert F. ; Hoelzer, Jacalyn D. ; Nazerian, Keyvan ; Bose, Henry R. / Avian reticuloendotheliosis virus : Identification of the hematopoietic target cell for transformation. In: Cell. 1981 ; Vol. 25, No. 2. pp. 421-431.
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