Axonal supercharged interpositional jump-graft with a hybrid artificial nerve conduit containing adipose-derived stem cells in facial nerve paresis rat model

Wataru Kamei, Hajime Matsumine, Hironobu Osaki, Yoshifumi Ueta, Satoshi Tsunoda, Mari Shimizu, Kazuki Hashimoto, Yosuke Niimi, Mariko Miyata, Hiroyuki Sakurai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: Interpositional jump-graft (IPJG) technique with the hypoglossal nerve for supercharging can be applied in a facial nerve paresis case. In IPJG, an autologous nerve is required, and the donor site morbidity is unavoidable. Biodegradable nerve conduits are made from polyglycolic acid (PGA) and used recently without donor site complications after providing autologous grafts. Hybrid artificial nerve conduits with adipose-derived stem cells (ASCs) also attract attention as a nerve-regeneration enhancing agent. This study examined the effect of hybrid artificial nerve conduit on IPJG. Materials and methods: A total of 34 Lewis rats were used and divided into 4 groups by the bridge materials: autograft (n = 8), PGA nerve conduit (n = 8), hybrid PGA nerve conduit with ASCs (n = 8), and the nontreated control groups (n = 8). ASCs were collected from 2 rats and cultured. The animals were assessed physiologically and histopathologically at 13 weeks after surgery. Results: In compound muscle action potential, the amplitude of hybrid PGA group (3,222 ± 1,779 μV) was significantly higher than that of PGA group (1,961 ± 445 μV, P <.05), and no significant difference between hybrid PGA and autograft group. All treated groups showed a myelinated nerve regeneration with double innervation in hypoglossal and facial nerve nuclei for vibrissal muscle. Conclusion: This study showed the effectiveness of IPJG with a hybrid PGA conduit especially in physiological examination.

Original languageEnglish (US)
Pages (from-to)889-898
Number of pages10
JournalMicrosurgery
Volume38
Issue number8
DOIs
StatePublished - Nov 1 2018
Externally publishedYes

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Polyglycolic Acid
Facial Paralysis
Facial Nerve
Stem Cells
Transplants
Hypoglossal Nerve
Nerve Regeneration
Autografts
Muscles
Action Potentials
Morbidity
Control Groups

ASJC Scopus subject areas

  • Surgery

Cite this

Axonal supercharged interpositional jump-graft with a hybrid artificial nerve conduit containing adipose-derived stem cells in facial nerve paresis rat model. / Kamei, Wataru; Matsumine, Hajime; Osaki, Hironobu; Ueta, Yoshifumi; Tsunoda, Satoshi; Shimizu, Mari; Hashimoto, Kazuki; Niimi, Yosuke; Miyata, Mariko; Sakurai, Hiroyuki.

In: Microsurgery, Vol. 38, No. 8, 01.11.2018, p. 889-898.

Research output: Contribution to journalArticle

Kamei, W, Matsumine, H, Osaki, H, Ueta, Y, Tsunoda, S, Shimizu, M, Hashimoto, K, Niimi, Y, Miyata, M & Sakurai, H 2018, 'Axonal supercharged interpositional jump-graft with a hybrid artificial nerve conduit containing adipose-derived stem cells in facial nerve paresis rat model', Microsurgery, vol. 38, no. 8, pp. 889-898. https://doi.org/10.1002/micr.30389
Kamei, Wataru ; Matsumine, Hajime ; Osaki, Hironobu ; Ueta, Yoshifumi ; Tsunoda, Satoshi ; Shimizu, Mari ; Hashimoto, Kazuki ; Niimi, Yosuke ; Miyata, Mariko ; Sakurai, Hiroyuki. / Axonal supercharged interpositional jump-graft with a hybrid artificial nerve conduit containing adipose-derived stem cells in facial nerve paresis rat model. In: Microsurgery. 2018 ; Vol. 38, No. 8. pp. 889-898.
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AU - Matsumine, Hajime

AU - Osaki, Hironobu

AU - Ueta, Yoshifumi

AU - Tsunoda, Satoshi

AU - Shimizu, Mari

AU - Hashimoto, Kazuki

AU - Niimi, Yosuke

AU - Miyata, Mariko

AU - Sakurai, Hiroyuki

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