B cell production of tumor necrosis factor in response to pneumocystis murina infection in mice

Michael M. Opata, Zhan Ye, Melissa Hollifield, Beth A. Garvy

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Pneumocystis species are opportunistic fungal pathogens that induce tumor necrosis factor (TNF) production by alveolar macrophages. Here we report that B cells from the draining lymph nodes as well as lung CD4+ T cells are important producers of TNF upon Pneumocystis murina infection. To determine the importance of B cell-derived TNF in the primary response to P. murina, we generated bone marrow chimeras whose B cells were unable to produce TNF. The lung P. murina burden at 10 days postinfection in TNF knockout (TNFKO) chimeras was significantly higher than that in wild-type (WT) chimeras, which corresponded to reduced numbers of activated CD4+ T cells in the lungs at this early time point. Furthermore, CD4+ T cells isolated from P. murina-infected TNFKO chimeras were unable to stimulate clearance of P. murina upon adoptive transfer to recombinase-deficient (RAG1KO) hosts. Together, these data indicate that B cell-derived TNF plays an important function in promoting CD4+ T cell expansion and production of TNF and facilitating protection against P. murina infection.

Original languageEnglish (US)
Pages (from-to)4252-4260
Number of pages9
JournalInfection and Immunity
Volume81
Issue number11
DOIs
StatePublished - Nov 2013

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Pneumocystis Infections
Murinae
B-Lymphocytes
Tumor Necrosis Factor-alpha
T-Lymphocytes
Lung
Pneumocystis
Recombinases
Adoptive Transfer
Alveolar Macrophages
Lymph Nodes
Bone Marrow
Infection

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

B cell production of tumor necrosis factor in response to pneumocystis murina infection in mice. / Opata, Michael M.; Ye, Zhan; Hollifield, Melissa; Garvy, Beth A.

In: Infection and Immunity, Vol. 81, No. 11, 11.2013, p. 4252-4260.

Research output: Contribution to journalArticle

Opata, Michael M. ; Ye, Zhan ; Hollifield, Melissa ; Garvy, Beth A. / B cell production of tumor necrosis factor in response to pneumocystis murina infection in mice. In: Infection and Immunity. 2013 ; Vol. 81, No. 11. pp. 4252-4260.
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