B lymphocytes are required during the early priming of CD4+ T cells for clearance of pneumocystis infection in mice

Michael M. Opata, Melissa L. Hollifield, Frances E. Lund, Troy D. Randall, Robert Dunn, Beth A. Garvy, David J. Feola

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

B cells play a critical role in the clearance of Pneumocystis. In addition to production of Pneumocystis-specific Abs, B cells are required during the priming phase for CD4+ T cells to expand normally and generate memory. Clearance of Pneumocystis was found to be dependent on Ag specific B cells and on the ability of B cells to secrete Pneumocystis-specific Ab, as mice with B cells defective in these functions or with a restricted BCR were unable to control Pneumocystis infection. Because Pneumocystis-specific antiserum was only able to partially protect B cell-deficient mice from infection, we hypothesized that optimal T cell priming requires fully functional B cells. Using adoptive transfer and B cell depletion strategies, we determined that optimal priming of CD4+ T cells requires B cells during the first 2-3 d of infection and that this was independent of the production of Ab. T cells that were removed from Pneumocystis-infected mice during the priming phase were fully functional and able to clear Pneumocystis infection upon adoptive transfer into Rag1-/- hosts, but this effect was ablated in mice that lacked fully functional B cells. Our results indicate that T cell priming requires a complete environment of Ag presentation and activation signals to become fully functional in this model of Pneumocystis infection.

Original languageEnglish (US)
Pages (from-to)611-620
Number of pages10
JournalJournal of Immunology
Volume195
Issue number2
DOIs
StatePublished - Jul 15 2015

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Opata, M. M., Hollifield, M. L., Lund, F. E., Randall, T. D., Dunn, R., Garvy, B. A., & Feola, D. J. (2015). B lymphocytes are required during the early priming of CD4+ T cells for clearance of pneumocystis infection in mice. Journal of Immunology, 195(2), 611-620. https://doi.org/10.4049/jimmunol.1500112