Bacillus cereus induces permeability of an in vitro blood-retina barrier

A. L. Moyer, R. T. Ramadan, J. Thurman, A. Burroughs, M. C. Callegan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Most Bacillus cereus toxin production is controlled by the quorum-sensing-dependent, pleiotropic global regulator plcR, which contributes to the organism's virulence in the eye. The purpose of this study was to analyze the effects of B. cereus infection and plcR-regulated toxins on the barrier function of retinal pigment epithelium (RPE) cells, the primary cells of the blood-retina barrier. Human ARPE-19 cells were apically inoculated with wild-type or quorum-sensing-deficient B. cereus, and cytotoxicity was analyzed. plcR-regulated toxins were not required for B. cereus-induced RPE cytotoxicity, but these toxins did increase the rate of cell death, primarily by necrosis. B. cereus infection of polarized RPE cell monolayers resulted in increased barrier permeability, independent of plcR-regulated toxins. Loss of both occludin and ZO-1 expression occurred by 8 h postinfection, but alterations in tight junctions appeared to precede cytotoxicity. Of the several proinflammatory cytokines analyzed, only interleukin-6 was produced in response to B. cereus infection. These results demonstrate the deleterious effects of B. cereus infection on RPE barrier function and suggest that plcR-regulated toxins may not contribute significantly to RPE barrier permeability during infection.

Original languageEnglish (US)
Pages (from-to)1358-1367
Number of pages10
JournalInfection and Immunity
Volume76
Issue number4
DOIs
StatePublished - Apr 2008
Externally publishedYes

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Bacillus cereus
Retina
Permeability
Retinal Pigment Epithelium
Quorum Sensing
Infection
Occludin
Tight Junctions
In Vitro Techniques
Virulence
Interleukin-6
Blood Cells
Cell Death
Necrosis
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Moyer, A. L., Ramadan, R. T., Thurman, J., Burroughs, A., & Callegan, M. C. (2008). Bacillus cereus induces permeability of an in vitro blood-retina barrier. Infection and Immunity, 76(4), 1358-1367. https://doi.org/10.1128/IAI.01330-07

Bacillus cereus induces permeability of an in vitro blood-retina barrier. / Moyer, A. L.; Ramadan, R. T.; Thurman, J.; Burroughs, A.; Callegan, M. C.

In: Infection and Immunity, Vol. 76, No. 4, 04.2008, p. 1358-1367.

Research output: Contribution to journalArticle

Moyer, AL, Ramadan, RT, Thurman, J, Burroughs, A & Callegan, MC 2008, 'Bacillus cereus induces permeability of an in vitro blood-retina barrier', Infection and Immunity, vol. 76, no. 4, pp. 1358-1367. https://doi.org/10.1128/IAI.01330-07
Moyer AL, Ramadan RT, Thurman J, Burroughs A, Callegan MC. Bacillus cereus induces permeability of an in vitro blood-retina barrier. Infection and Immunity. 2008 Apr;76(4):1358-1367. https://doi.org/10.1128/IAI.01330-07
Moyer, A. L. ; Ramadan, R. T. ; Thurman, J. ; Burroughs, A. ; Callegan, M. C. / Bacillus cereus induces permeability of an in vitro blood-retina barrier. In: Infection and Immunity. 2008 ; Vol. 76, No. 4. pp. 1358-1367.
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