Backscattered electron imaging and energy-dispersive x-ray microanalysis studies of evidence for calcium salt heterogeneity in fifteen gallstones from an elderly human

T. Kodaka, R. Mori, K. Debari, R. Takiguchi, S. Higashi, H. S. Kaufman, K. D. Lillemoe, R. D. Soloway, U. Wosiewitz, O. Johari

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We examined 15 variably-sized gallstones, taken from an elderly male, by backscattered electron imaging and energy-dispersive X-ray microanalysis to learn the structural and distribution patterns of gallstone calcium (Ca-) salts. Of the 13 cholesterol-rich stones, nine stones had peripheral concentric layers of Ca-carbonate, whereas 2 stones had peripheral layers of Ca-phosphate. No Ca-salts were detected from 2 cholesterol-rich stones. The 2 stones containing Ca-phosphate had no Ca-salt cores, whereas the stones containing Ca-carbonate were separated into 3 different types: two stones with a Ca-carbonate core, four stones with several Ca-bilirubinate cores of glass-like structure, and 3 stones lacking Ca-salt cores. A closer view of the Ca-salt layers, which may be occasionally coexistent with Ca- bilirubinate, mainly showed either laminate deposits or numerous globules with a few laminae. Of the 2 cholesterol-poor stones, one had dispersed particles mainly of Ca-phosphate, and the other had loosely dispersed particles with small amounts of Ca-phosphate, bilirubinate, and/or palmitate. Some relationship between the size and Ca-salt species of these gallstones was suggested. Gallstones collected from the same individual showed a considerable heterogeneity of Ca-salts.

Original languageEnglish (US)
Pages (from-to)907-924
Number of pages18
JournalScanning Microscopy
Volume9
Issue number3
StatePublished - Dec 1 1995

Keywords

  • Gallstones
  • backscattered electron imaging
  • calcium salts
  • distribution
  • energy-dispersive X-ray microanalysis
  • scanning electron microscopy
  • structure

ASJC Scopus subject areas

  • Instrumentation

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    Kodaka, T., Mori, R., Debari, K., Takiguchi, R., Higashi, S., Kaufman, H. S., Lillemoe, K. D., Soloway, R. D., Wosiewitz, U., & Johari, O. (1995). Backscattered electron imaging and energy-dispersive x-ray microanalysis studies of evidence for calcium salt heterogeneity in fifteen gallstones from an elderly human. Scanning Microscopy, 9(3), 907-924.