Bacterial flagellin is a dominant antigen in Crohn disease

Michael J. Lodes, Yingzi Cong, Charles O. Elson, Raodoh Mohamath, Carol J. Landers, Stephan R. Targan, Madeline Fort, Robert M. Hershberg

Research output: Contribution to journalArticle

592 Citations (Scopus)

Abstract

Chronic intestinal inflammation, as seen in inflammatory bowel disease (IBD), results from an aberrant and poorly understood mucosal immune response to the microbiota of the gastrointestinal tract in genetically susceptible individuals. Here we used serological expression cloning to identify commensal bacterial proteins that could, contribute to the pathogenesis of IBD. The dominant antigens identified were flagellins, molecules known to activate innate immunity via Toll-like receptor 5 (TLR5), and critical targets of the acquired immune system in host defense. Multiple strains of colitic mice had elevated serum anti-flagellin IgG2a responses and Th1 T cell responses to flagellin. In addition, flagellin-specific CD4+ T cells induced severe colitis when adoptively transferred into naive SCID mice. Serum IgG to these flagellins, but not to the dissimilar Salmonella muenchen flagellin, was elevated in patients with Crohn disease, but not in patients with ulcerative colitis or in controls. These results identify flagellins as a class of immunodominant antigens that stimulate pathogenic intestinal immune reactions in genetically diverse hosts and suggest new avenues for the diagnosis and antigen-directed therapy of patients with IBD.

Original languageEnglish (US)
Pages (from-to)1296-1306
Number of pages11
JournalJournal of Clinical Investigation
Volume113
Issue number9
DOIs
StatePublished - May 2004
Externally publishedYes

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Flagellin
Crohn Disease
Antigens
Inflammatory Bowel Diseases
Toll-Like Receptor 5
T-Lymphocytes
Immunodominant Epitopes
Mucosal Immunity
Th1 Cells
Bacterial Proteins
SCID Mice
Microbiota
Colitis
Serum
Ulcerative Colitis
Innate Immunity
Salmonella
Gastrointestinal Tract
Organism Cloning
Immune System

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Lodes, M. J., Cong, Y., Elson, C. O., Mohamath, R., Landers, C. J., Targan, S. R., ... Hershberg, R. M. (2004). Bacterial flagellin is a dominant antigen in Crohn disease. Journal of Clinical Investigation, 113(9), 1296-1306. https://doi.org/10.1172/JCI200420295

Bacterial flagellin is a dominant antigen in Crohn disease. / Lodes, Michael J.; Cong, Yingzi; Elson, Charles O.; Mohamath, Raodoh; Landers, Carol J.; Targan, Stephan R.; Fort, Madeline; Hershberg, Robert M.

In: Journal of Clinical Investigation, Vol. 113, No. 9, 05.2004, p. 1296-1306.

Research output: Contribution to journalArticle

Lodes, MJ, Cong, Y, Elson, CO, Mohamath, R, Landers, CJ, Targan, SR, Fort, M & Hershberg, RM 2004, 'Bacterial flagellin is a dominant antigen in Crohn disease', Journal of Clinical Investigation, vol. 113, no. 9, pp. 1296-1306. https://doi.org/10.1172/JCI200420295
Lodes MJ, Cong Y, Elson CO, Mohamath R, Landers CJ, Targan SR et al. Bacterial flagellin is a dominant antigen in Crohn disease. Journal of Clinical Investigation. 2004 May;113(9):1296-1306. https://doi.org/10.1172/JCI200420295
Lodes, Michael J. ; Cong, Yingzi ; Elson, Charles O. ; Mohamath, Raodoh ; Landers, Carol J. ; Targan, Stephan R. ; Fort, Madeline ; Hershberg, Robert M. / Bacterial flagellin is a dominant antigen in Crohn disease. In: Journal of Clinical Investigation. 2004 ; Vol. 113, No. 9. pp. 1296-1306.
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