Bacterial infections in Myd88-deficient mice

Jason S. Villano, Rong Fang, Timothy K. Cooper

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Three breeding colonies of Myd88-/- mice had a history of significant morbidity and mortality. Although strain-specific poor reproductive performance might explain neonatal death and dystocia, mice were found dead or required euthanasia because of moribundity, distended abdomen, head tilt, and seizures. Histopathology results included bacteremia, placentitis, metritis, peritonitis with abscess formation, and suppurative meningoencephalitis. Intralesional gram-negative coccobacilli were present, often in extremely high number. Cultures of samples of the cardiac blood of a mouse and from water-bottle sipper tubes provided to some affected mice grew Pseudomonas aeruginosa. In addition, affected tissues from 2 mice and feces from a third tested PCR-positive for P. aeruginosa. Although the mice had received autoclaved reverse-osmosis-purified drinking water, we suspect that the mice were inoculated with P. aeruginosa through contaminated sipper tubes. Because of the deficiency in most of the Toll-like receptor signaling pathways, these Myd88-/- mice were unlikely to have developed competitive innate and adaptive immune responses, resulting in bacterial infections. These clinical cases underscore the importance of understanding how genotype, phenotype and environment affect animal health. Sound husbandry and experimental practices are needed to prevent the exposure of immunodeficient mice to pathogens.

Original languageEnglish (US)
Pages (from-to)110-114
Number of pages5
JournalComparative Medicine
Volume64
Issue number2
StatePublished - 2014

Fingerprint

Toll-Like Receptors
Reverse osmosis
Bottles
Pathogens
bacterial infections
Bacterial Infections
Drinking Water
Animals
Blood
Health
Acoustic waves
Tissue
Water
mice
Pseudomonas aeruginosa
Osmosis
reverse osmosis
Dystocia
bacteremia
Meningoencephalitis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)
  • Medicine(all)

Cite this

Villano, J. S., Fang, R., & Cooper, T. K. (2014). Bacterial infections in Myd88-deficient mice. Comparative Medicine, 64(2), 110-114.

Bacterial infections in Myd88-deficient mice. / Villano, Jason S.; Fang, Rong; Cooper, Timothy K.

In: Comparative Medicine, Vol. 64, No. 2, 2014, p. 110-114.

Research output: Contribution to journalArticle

Villano, JS, Fang, R & Cooper, TK 2014, 'Bacterial infections in Myd88-deficient mice', Comparative Medicine, vol. 64, no. 2, pp. 110-114.
Villano, Jason S. ; Fang, Rong ; Cooper, Timothy K. / Bacterial infections in Myd88-deficient mice. In: Comparative Medicine. 2014 ; Vol. 64, No. 2. pp. 110-114.
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