Bacterial Modulation of Human Fetal Membrane Toll-like Receptor Expression

Vikki M. Abrahams, Julie A. Potter, Geeta Bhat, Morgan R. Peltier, George Saade, Ramkumar Menon

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Problem: Preterm premature rupture of fetal membranes (pPROM) occurs in 30-40% of spontaneous preterm births (PTB) and is associated with intra-amniotic infection and inflammation. The membranes may sense and respond to microbes via Toll-like receptors (TLRs); however, little is known about their expression and regulation in this tissue. The objective of this study was to evaluate the expression of TLRs 1-10 in fetal membranes after exposure to pathogens associated with intra-amniotic infection and PTB. Method of study: Normal human term fetal membrane explants were exposed to various bacteria. After 24 hrs, RNA was extracted and quantitative RT-PCR performed for TLRs1-10. Results: Treatment of fetal membranes with Mycoplasma hominis increased expression of TLR4, TLR6, and TLR8 mRNA. Ureaplasma parvum upregulated TLR8 mRNA, and Porphyromonas gingivalis significantly increased fetal membrane TLR7 expression. In contrast, treatment with Gram-negative Escherichia coli (and its cell wall component lipopolysaccharide) downregulated TLR10 mRNA. No effect was detected for Ureaplasma urealyticum, Gardnerella vaginalis, or Group B Streptococcus. Conclusion: These findings demonstrate that different types of bacteria have distinct effects on fetal membrane TLR expression patterns. Moreover, these findings highlight the disparity of fetal membrane responses to infection and thus suggest heterogeneity in the mechanisms by which infection-associated pregnancy complications, such as pPROM and PTB, arise.

Original languageEnglish (US)
Pages (from-to)33-40
Number of pages8
JournalAmerican Journal of Reproductive Immunology
Volume69
Issue number1
DOIs
StatePublished - Jan 2013

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Extraembryonic Membranes
Toll-Like Receptors
Premature Birth
Premature Rupture Fetal Membranes
Infection
Messenger RNA
Toll-Like Receptor 10
Toll-Like Receptor 1
Gardnerella vaginalis
Mycoplasma hominis
Ureaplasma
Ureaplasma urealyticum
Bacteria
Porphyromonas gingivalis
Streptococcus agalactiae
Pregnancy Complications
Cellular Structures
Cell Wall
Lipopolysaccharides
Down-Regulation

Keywords

  • Bacteria
  • Chorioamnion
  • Infection
  • Preterm birth
  • Toll-like receptor

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Bacterial Modulation of Human Fetal Membrane Toll-like Receptor Expression. / Abrahams, Vikki M.; Potter, Julie A.; Bhat, Geeta; Peltier, Morgan R.; Saade, George; Menon, Ramkumar.

In: American Journal of Reproductive Immunology, Vol. 69, No. 1, 01.2013, p. 33-40.

Research output: Contribution to journalArticle

Abrahams, Vikki M. ; Potter, Julie A. ; Bhat, Geeta ; Peltier, Morgan R. ; Saade, George ; Menon, Ramkumar. / Bacterial Modulation of Human Fetal Membrane Toll-like Receptor Expression. In: American Journal of Reproductive Immunology. 2013 ; Vol. 69, No. 1. pp. 33-40.
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