Bacterial toxicity of potassium tellurite: Unveiling an ancient enigma

José M. Pérez, Iván L. Calderón, Felipe A. Arenas, Derie E. Fuentes, Gonzalo A. Pradenas, Eugenia L. Fuentes, Juan M. Sandoval, Miguel E. Castro, Alex O. Elías, Claudio C. Vásquez

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Biochemical, genetic, enzymatic and molecular approaches were used to demonstrate, for the first time, that tellurite (TeO3 2-) toxicity in E. coli involves superoxide formation. This radical is derived, at least in part, from enzymatic TeO3 2- reduction. This conclusion is supported by the following observations made in K2TeO3-treated E. coli BW25113: i) induction of the ibpA gene encoding for the small heat shock protein IbpA, which has been associated with resistance to superoxide, ii) increase of cytoplasmic reactive oxygen species (ROS) as determined with ROS-specific probe 2′7′-dichlorodihydrofluorescein diacetate (H2DCFDA), iii) increase of carbonyl content in cellular proteins, iv) increase in the generation of thiobarbituric acid-reactive substances (TBARs), v) inactivation of oxidative stress-sensitive [Fe-S] enzymes such as aconitase, vi) increase of superoxide dismutase (SOD) activity, vii) increase of sodA, sodB and soxS mRNA transcription, and viii) generation of superoxide radical during in vitro enzymatic reduction of potassium tellurite.

Original languageEnglish (US)
Article numbere211
JournalPLoS One
Volume2
Issue number2
DOIs
StatePublished - Feb 14 2007
Externally publishedYes

Fingerprint

Superoxides
superoxide anion
Toxicity
potassium
toxicity
Escherichia coli
reactive oxygen species
Reactive Oxygen Species
Aconitate Hydratase
Small Heat-Shock Proteins
aconitate hydratase
Gene encoding
Oxidative stress
Thiobarbituric Acid Reactive Substances
gene induction
Transcription
heat shock proteins
thiobarbituric acid-reactive substances
molecular genetics
Superoxide Dismutase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Pérez, J. M., Calderón, I. L., Arenas, F. A., Fuentes, D. E., Pradenas, G. A., Fuentes, E. L., ... Vásquez, C. C. (2007). Bacterial toxicity of potassium tellurite: Unveiling an ancient enigma. PLoS One, 2(2), [e211]. https://doi.org/10.1371/journal.pone.0000211

Bacterial toxicity of potassium tellurite : Unveiling an ancient enigma. / Pérez, José M.; Calderón, Iván L.; Arenas, Felipe A.; Fuentes, Derie E.; Pradenas, Gonzalo A.; Fuentes, Eugenia L.; Sandoval, Juan M.; Castro, Miguel E.; Elías, Alex O.; Vásquez, Claudio C.

In: PLoS One, Vol. 2, No. 2, e211, 14.02.2007.

Research output: Contribution to journalArticle

Pérez, JM, Calderón, IL, Arenas, FA, Fuentes, DE, Pradenas, GA, Fuentes, EL, Sandoval, JM, Castro, ME, Elías, AO & Vásquez, CC 2007, 'Bacterial toxicity of potassium tellurite: Unveiling an ancient enigma', PLoS One, vol. 2, no. 2, e211. https://doi.org/10.1371/journal.pone.0000211
Pérez JM, Calderón IL, Arenas FA, Fuentes DE, Pradenas GA, Fuentes EL et al. Bacterial toxicity of potassium tellurite: Unveiling an ancient enigma. PLoS One. 2007 Feb 14;2(2). e211. https://doi.org/10.1371/journal.pone.0000211
Pérez, José M. ; Calderón, Iván L. ; Arenas, Felipe A. ; Fuentes, Derie E. ; Pradenas, Gonzalo A. ; Fuentes, Eugenia L. ; Sandoval, Juan M. ; Castro, Miguel E. ; Elías, Alex O. ; Vásquez, Claudio C. / Bacterial toxicity of potassium tellurite : Unveiling an ancient enigma. In: PLoS One. 2007 ; Vol. 2, No. 2.
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