Bacterial translocation in burned mice after administration of various diets including fiber- and glutamine-enriched enteral formulas

Ramon Zapata Sirvent, J. F. Hansbrough, M. M. Ohara, M. Rice-Asaro, W. L. Nyhan

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Objective: Severe burn injury can produce acute gastrointestinal derangements which may facilitate bacterial translocation to mesenteric lymph nodes. We studied the effects of feeding different dietary formulations on bacterial translocation in burned mice. Design: Prospective, blinded, nonrandomized laboratory study. Setting: Research laboratory. Subjects: One hundred sixty-nine female, outbred, CF-1 mice, 8 to 12 wks of age. Interventions: Anesthetized mice received a 32% total body surface area, full-thickness burn injury. Mice were then fed with: a) mouse chow; b) a low- residue enteral formula; c) a high-protein, high-fat enteral formula; d) an enteral formula with high concentrations of supplemental glutamine; or e) an enteral formula that contains soy fiber. Measurements and Main Results: Burned mice that were fed the low-residue enteral formula demonstrated increased mortality rate (21.2%, p = .05) compared with chow-fed mice in the 2-day postburn period (0 mortality); other burn-diet groups had intermediate mortality rates. In surviving mice, bacterial translocation was found to be: a) lowest in the group fed chow (31.0%) and the high glutamine formula (30.8%); b) intermediate in the group fed formula and soy fiber (44.8%, NS compared with burn-chow group); and c) highest in the group receiving the low-residue enteral formula (73.1%, p < .005) and high-protein, high-fat enteral formula (59.3%, p < .05). Conclusions: Dietary composition markedly affects bacterial translocation in this animal burn model. Commercial enteral diets containing fiber and high concentrations of glutamine provide protection for the gut after burn injury and reduce the occurrence of bacterial translocation in this animal model.

Original languageEnglish (US)
Pages (from-to)690-696
Number of pages7
JournalCritical Care Medicine
Volume22
Issue number4
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

Bacterial Translocation
Glutamine
Small Intestine
Diet
Burns
Mortality
Wounds and Injuries
Animal Models
Fats
Formulated Food
Body Surface Area
Proteins
Lymph Nodes

Keywords

  • burn
  • dietary formulation
  • gastrointestinal diseases
  • glutamine
  • nutrition, enteral
  • shock
  • translocation, bacterial

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Bacterial translocation in burned mice after administration of various diets including fiber- and glutamine-enriched enteral formulas. / Zapata Sirvent, Ramon; Hansbrough, J. F.; Ohara, M. M.; Rice-Asaro, M.; Nyhan, W. L.

In: Critical Care Medicine, Vol. 22, No. 4, 01.01.1994, p. 690-696.

Research output: Contribution to journalArticle

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abstract = "Objective: Severe burn injury can produce acute gastrointestinal derangements which may facilitate bacterial translocation to mesenteric lymph nodes. We studied the effects of feeding different dietary formulations on bacterial translocation in burned mice. Design: Prospective, blinded, nonrandomized laboratory study. Setting: Research laboratory. Subjects: One hundred sixty-nine female, outbred, CF-1 mice, 8 to 12 wks of age. Interventions: Anesthetized mice received a 32{\%} total body surface area, full-thickness burn injury. Mice were then fed with: a) mouse chow; b) a low- residue enteral formula; c) a high-protein, high-fat enteral formula; d) an enteral formula with high concentrations of supplemental glutamine; or e) an enteral formula that contains soy fiber. Measurements and Main Results: Burned mice that were fed the low-residue enteral formula demonstrated increased mortality rate (21.2{\%}, p = .05) compared with chow-fed mice in the 2-day postburn period (0 mortality); other burn-diet groups had intermediate mortality rates. In surviving mice, bacterial translocation was found to be: a) lowest in the group fed chow (31.0{\%}) and the high glutamine formula (30.8{\%}); b) intermediate in the group fed formula and soy fiber (44.8{\%}, NS compared with burn-chow group); and c) highest in the group receiving the low-residue enteral formula (73.1{\%}, p < .005) and high-protein, high-fat enteral formula (59.3{\%}, p < .05). Conclusions: Dietary composition markedly affects bacterial translocation in this animal burn model. Commercial enteral diets containing fiber and high concentrations of glutamine provide protection for the gut after burn injury and reduce the occurrence of bacterial translocation in this animal model.",
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AU - Rice-Asaro, M.

AU - Nyhan, W. L.

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N2 - Objective: Severe burn injury can produce acute gastrointestinal derangements which may facilitate bacterial translocation to mesenteric lymph nodes. We studied the effects of feeding different dietary formulations on bacterial translocation in burned mice. Design: Prospective, blinded, nonrandomized laboratory study. Setting: Research laboratory. Subjects: One hundred sixty-nine female, outbred, CF-1 mice, 8 to 12 wks of age. Interventions: Anesthetized mice received a 32% total body surface area, full-thickness burn injury. Mice were then fed with: a) mouse chow; b) a low- residue enteral formula; c) a high-protein, high-fat enteral formula; d) an enteral formula with high concentrations of supplemental glutamine; or e) an enteral formula that contains soy fiber. Measurements and Main Results: Burned mice that were fed the low-residue enteral formula demonstrated increased mortality rate (21.2%, p = .05) compared with chow-fed mice in the 2-day postburn period (0 mortality); other burn-diet groups had intermediate mortality rates. In surviving mice, bacterial translocation was found to be: a) lowest in the group fed chow (31.0%) and the high glutamine formula (30.8%); b) intermediate in the group fed formula and soy fiber (44.8%, NS compared with burn-chow group); and c) highest in the group receiving the low-residue enteral formula (73.1%, p < .005) and high-protein, high-fat enteral formula (59.3%, p < .05). Conclusions: Dietary composition markedly affects bacterial translocation in this animal burn model. Commercial enteral diets containing fiber and high concentrations of glutamine provide protection for the gut after burn injury and reduce the occurrence of bacterial translocation in this animal model.

AB - Objective: Severe burn injury can produce acute gastrointestinal derangements which may facilitate bacterial translocation to mesenteric lymph nodes. We studied the effects of feeding different dietary formulations on bacterial translocation in burned mice. Design: Prospective, blinded, nonrandomized laboratory study. Setting: Research laboratory. Subjects: One hundred sixty-nine female, outbred, CF-1 mice, 8 to 12 wks of age. Interventions: Anesthetized mice received a 32% total body surface area, full-thickness burn injury. Mice were then fed with: a) mouse chow; b) a low- residue enteral formula; c) a high-protein, high-fat enteral formula; d) an enteral formula with high concentrations of supplemental glutamine; or e) an enteral formula that contains soy fiber. Measurements and Main Results: Burned mice that were fed the low-residue enteral formula demonstrated increased mortality rate (21.2%, p = .05) compared with chow-fed mice in the 2-day postburn period (0 mortality); other burn-diet groups had intermediate mortality rates. In surviving mice, bacterial translocation was found to be: a) lowest in the group fed chow (31.0%) and the high glutamine formula (30.8%); b) intermediate in the group fed formula and soy fiber (44.8%, NS compared with burn-chow group); and c) highest in the group receiving the low-residue enteral formula (73.1%, p < .005) and high-protein, high-fat enteral formula (59.3%, p < .05). Conclusions: Dietary composition markedly affects bacterial translocation in this animal burn model. Commercial enteral diets containing fiber and high concentrations of glutamine provide protection for the gut after burn injury and reduce the occurrence of bacterial translocation in this animal model.

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