Bacteriophage φ29 scaffolding protein gp7 before and after prohead assembly

Marc Morais, Shuji Kanamarul, Mohammed O. Badasso, Jaya S. Koti, Barbara A L Owen, Cynthia T. McMurray, Dwight L. Anderson, Michael G. Rossmann

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Three-dimensional structures of the double-stranded DNA bacteriophage φ29 scaffolding protein (gp7) before and after prohead assembly have been determined at resolutions of 2.2 and 2.8 Å, respectively. Both structures are dimers that resemble arrows, with a four-helix bundle composing the arrowhead and a coiled coil forming the tail. The structural resemblance of gp7 to the yeast transcription factor GCN4 suggests a DNA-binding function that was confirmed by native gel electrophoresis. DNA binding to gp7 may have a role in mediating the structural transition from prohead to mature virus and scaffold release. A cryo-EM analysis indicates that gp7 is arranged inside the capsid as a series of concentric shells. The position of the higher density features in these shells correlates with the positions of hexamers in the equatorial region of the capsid, suggesting that gp7 may regulate formation of the prolate head through interactions with these hexamers.

Original languageEnglish (US)
Pages (from-to)572-576
Number of pages5
JournalNature Structural Biology
Volume10
Issue number7
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

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Bacteriophages
Capsid
DNA
Sagittaria
Phosmet
Virus Release
Proteins
Electrophoresis
Viruses
Scaffolds
Dimers
Yeast
Transcription Factors
Yeasts
Gels

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

Morais, M., Kanamarul, S., Badasso, M. O., Koti, J. S., Owen, B. A. L., McMurray, C. T., ... Rossmann, M. G. (2003). Bacteriophage φ29 scaffolding protein gp7 before and after prohead assembly. Nature Structural Biology, 10(7), 572-576. https://doi.org/10.1038/nsb939

Bacteriophage φ29 scaffolding protein gp7 before and after prohead assembly. / Morais, Marc; Kanamarul, Shuji; Badasso, Mohammed O.; Koti, Jaya S.; Owen, Barbara A L; McMurray, Cynthia T.; Anderson, Dwight L.; Rossmann, Michael G.

In: Nature Structural Biology, Vol. 10, No. 7, 01.07.2003, p. 572-576.

Research output: Contribution to journalArticle

Morais, M, Kanamarul, S, Badasso, MO, Koti, JS, Owen, BAL, McMurray, CT, Anderson, DL & Rossmann, MG 2003, 'Bacteriophage φ29 scaffolding protein gp7 before and after prohead assembly', Nature Structural Biology, vol. 10, no. 7, pp. 572-576. https://doi.org/10.1038/nsb939
Morais M, Kanamarul S, Badasso MO, Koti JS, Owen BAL, McMurray CT et al. Bacteriophage φ29 scaffolding protein gp7 before and after prohead assembly. Nature Structural Biology. 2003 Jul 1;10(7):572-576. https://doi.org/10.1038/nsb939
Morais, Marc ; Kanamarul, Shuji ; Badasso, Mohammed O. ; Koti, Jaya S. ; Owen, Barbara A L ; McMurray, Cynthia T. ; Anderson, Dwight L. ; Rossmann, Michael G. / Bacteriophage φ29 scaffolding protein gp7 before and after prohead assembly. In: Nature Structural Biology. 2003 ; Vol. 10, No. 7. pp. 572-576.
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