Bartonella melophagi in blood of domestic sheep (Ovis aries) and sheep keds (Melophagus ovinus) from the southwestern US: Cultures, genetic characterization, and ecological connections

Michael Kosoy, Ying Bai, Russell Enscore, Maria Rosales Rizzo, Scott Bender, Vsevolod Popov, Levent Albayrak, Yuriy Fofanov, Bruno Chomel

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Bartonella melophagi sp. nov. was isolated from domestic sheep blood and from sheep keds (Melophagus ovinus) from the southwestern United States. The sequence analyses of the reference strain performed by six molecular markers consistently demonstrated that B. melophagi relates to but differ from other Bartonella species isolated from domestic and wild ruminants. Presence of 183 genes specific for B. melophagi, being absent in genomes of other Bartonella species associated with ruminants also supports the separation of this bacterial species from species of other ruminants. Bartonella DNA was detected in all investigated sheep keds; however, culturing of these bacteria from sheep blood rejects a speculation that B. melophagi is an obligatory endosymbiont. Instead, the results support the hypothesis that the domestic sheep is a natural host reservoir for B. melophagi and the sheep ked its main vector. This bacterium was not isolated from the blood of bighorn sheep and domestic goats belonging to the same subfamily Caprinae. B. melophagi has also been shown to be zoonotic and needs to be investigated further.

Original languageEnglish (US)
Pages (from-to)43-49
Number of pages7
JournalVeterinary Microbiology
Volume190
DOIs
StatePublished - Jul 15 2016

Fingerprint

Melophagus ovinus
Hippobosca
Bartonella
Domestic Sheep
Sheep
Ruminants
sheep
blood
ruminants
Bighorn Sheep
Southwestern United States
Bacteria
Zoonoses
Ovis canadensis
disease reservoirs
Goats
bacteria
Sequence Analysis
endosymbionts
Genome

Keywords

  • Bartonellosis
  • Bighorn sheep
  • Domestic sheep
  • Genotyping
  • Sheep ked

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Bartonella melophagi in blood of domestic sheep (Ovis aries) and sheep keds (Melophagus ovinus) from the southwestern US : Cultures, genetic characterization, and ecological connections. / Kosoy, Michael; Bai, Ying; Enscore, Russell; Rizzo, Maria Rosales; Bender, Scott; Popov, Vsevolod; Albayrak, Levent; Fofanov, Yuriy; Chomel, Bruno.

In: Veterinary Microbiology, Vol. 190, 15.07.2016, p. 43-49.

Research output: Contribution to journalArticle

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