Base of the measles virus fusion trimer head receives the signal that triggers membrane fusion

Swapna Apte-Sengupta, Surendra Negi, Vincent H J Leonard, Numan Oezguen, Chanakha K. Navaratnarajah, Werner Braun, Roberto Cattaneo

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The measles virus (MV) fusion (F) protein trimer executes membrane fusion after receiving a signal elicited by receptor binding to the hemagglutinin (H) tetramer. Where and how this signal is received is understood neither for MV nor for other paramyxoviruses. Because only the prefusion structure of the parainfluenza virus 5 (PIV5) F-trimer is available, to study signal receipt by the MV F-trimer, we generated and energy-refined a homology model. We used two approaches to predict surface residues of the model interacting with other proteins. Both approaches measured interface propensity values for patches of residues. The second approach identified, in addition, individual residues based on the conservation of physical chemical properties among F-proteins. Altogether, about 50 candidate interactive residues were identified. Through iterative cycles of mutagenesis and functional analysis, we characterized six residues that are required specifically for signal transmission; their mutation interferes with fusion, although still allowing efficient F-protein processing and cell surface transport. One residue is located adjacent to the fusion peptide, four line a cavity in the base of the F-trimer head, while the sixth residue is located near this cavity. Hydrophobic interactions in the cavity sustain the fusion process and contacts with H. The cavity is flanked by two different subunits of the F-trimer. Tetrameric H-stalks may be lodged in apposed cavities of two F-trimers. Because these insights are based on a PIV5 homology model, the signal receipt mechanism may be conserved among paramyxoviruses.

Original languageEnglish (US)
Pages (from-to)33026-33035
Number of pages10
JournalJournal of Biological Chemistry
Volume287
Issue number39
DOIs
StatePublished - Sep 21 2012

Fingerprint

Measles virus
Membrane Fusion
Parainfluenza Virus 5
Viruses
Fusion reactions
Head
Membranes
Viral Fusion Proteins
Hemagglutinins
Hydrophobic and Hydrophilic Interactions
Proteins
Mutagenesis
Membrane Proteins
Functional analysis
Peptides
Mutation
Chemical properties
Conservation
Processing

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Apte-Sengupta, S., Negi, S., Leonard, V. H. J., Oezguen, N., Navaratnarajah, C. K., Braun, W., & Cattaneo, R. (2012). Base of the measles virus fusion trimer head receives the signal that triggers membrane fusion. Journal of Biological Chemistry, 287(39), 33026-33035. https://doi.org/10.1074/jbc.M112.373308

Base of the measles virus fusion trimer head receives the signal that triggers membrane fusion. / Apte-Sengupta, Swapna; Negi, Surendra; Leonard, Vincent H J; Oezguen, Numan; Navaratnarajah, Chanakha K.; Braun, Werner; Cattaneo, Roberto.

In: Journal of Biological Chemistry, Vol. 287, No. 39, 21.09.2012, p. 33026-33035.

Research output: Contribution to journalArticle

Apte-Sengupta, S, Negi, S, Leonard, VHJ, Oezguen, N, Navaratnarajah, CK, Braun, W & Cattaneo, R 2012, 'Base of the measles virus fusion trimer head receives the signal that triggers membrane fusion', Journal of Biological Chemistry, vol. 287, no. 39, pp. 33026-33035. https://doi.org/10.1074/jbc.M112.373308
Apte-Sengupta, Swapna ; Negi, Surendra ; Leonard, Vincent H J ; Oezguen, Numan ; Navaratnarajah, Chanakha K. ; Braun, Werner ; Cattaneo, Roberto. / Base of the measles virus fusion trimer head receives the signal that triggers membrane fusion. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 39. pp. 33026-33035.
@article{268a0fdee75e4c779af4644d3a275db5,
title = "Base of the measles virus fusion trimer head receives the signal that triggers membrane fusion",
abstract = "The measles virus (MV) fusion (F) protein trimer executes membrane fusion after receiving a signal elicited by receptor binding to the hemagglutinin (H) tetramer. Where and how this signal is received is understood neither for MV nor for other paramyxoviruses. Because only the prefusion structure of the parainfluenza virus 5 (PIV5) F-trimer is available, to study signal receipt by the MV F-trimer, we generated and energy-refined a homology model. We used two approaches to predict surface residues of the model interacting with other proteins. Both approaches measured interface propensity values for patches of residues. The second approach identified, in addition, individual residues based on the conservation of physical chemical properties among F-proteins. Altogether, about 50 candidate interactive residues were identified. Through iterative cycles of mutagenesis and functional analysis, we characterized six residues that are required specifically for signal transmission; their mutation interferes with fusion, although still allowing efficient F-protein processing and cell surface transport. One residue is located adjacent to the fusion peptide, four line a cavity in the base of the F-trimer head, while the sixth residue is located near this cavity. Hydrophobic interactions in the cavity sustain the fusion process and contacts with H. The cavity is flanked by two different subunits of the F-trimer. Tetrameric H-stalks may be lodged in apposed cavities of two F-trimers. Because these insights are based on a PIV5 homology model, the signal receipt mechanism may be conserved among paramyxoviruses.",
author = "Swapna Apte-Sengupta and Surendra Negi and Leonard, {Vincent H J} and Numan Oezguen and Navaratnarajah, {Chanakha K.} and Werner Braun and Roberto Cattaneo",
year = "2012",
month = "9",
day = "21",
doi = "10.1074/jbc.M112.373308",
language = "English (US)",
volume = "287",
pages = "33026--33035",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "39",

}

TY - JOUR

T1 - Base of the measles virus fusion trimer head receives the signal that triggers membrane fusion

AU - Apte-Sengupta, Swapna

AU - Negi, Surendra

AU - Leonard, Vincent H J

AU - Oezguen, Numan

AU - Navaratnarajah, Chanakha K.

AU - Braun, Werner

AU - Cattaneo, Roberto

PY - 2012/9/21

Y1 - 2012/9/21

N2 - The measles virus (MV) fusion (F) protein trimer executes membrane fusion after receiving a signal elicited by receptor binding to the hemagglutinin (H) tetramer. Where and how this signal is received is understood neither for MV nor for other paramyxoviruses. Because only the prefusion structure of the parainfluenza virus 5 (PIV5) F-trimer is available, to study signal receipt by the MV F-trimer, we generated and energy-refined a homology model. We used two approaches to predict surface residues of the model interacting with other proteins. Both approaches measured interface propensity values for patches of residues. The second approach identified, in addition, individual residues based on the conservation of physical chemical properties among F-proteins. Altogether, about 50 candidate interactive residues were identified. Through iterative cycles of mutagenesis and functional analysis, we characterized six residues that are required specifically for signal transmission; their mutation interferes with fusion, although still allowing efficient F-protein processing and cell surface transport. One residue is located adjacent to the fusion peptide, four line a cavity in the base of the F-trimer head, while the sixth residue is located near this cavity. Hydrophobic interactions in the cavity sustain the fusion process and contacts with H. The cavity is flanked by two different subunits of the F-trimer. Tetrameric H-stalks may be lodged in apposed cavities of two F-trimers. Because these insights are based on a PIV5 homology model, the signal receipt mechanism may be conserved among paramyxoviruses.

AB - The measles virus (MV) fusion (F) protein trimer executes membrane fusion after receiving a signal elicited by receptor binding to the hemagglutinin (H) tetramer. Where and how this signal is received is understood neither for MV nor for other paramyxoviruses. Because only the prefusion structure of the parainfluenza virus 5 (PIV5) F-trimer is available, to study signal receipt by the MV F-trimer, we generated and energy-refined a homology model. We used two approaches to predict surface residues of the model interacting with other proteins. Both approaches measured interface propensity values for patches of residues. The second approach identified, in addition, individual residues based on the conservation of physical chemical properties among F-proteins. Altogether, about 50 candidate interactive residues were identified. Through iterative cycles of mutagenesis and functional analysis, we characterized six residues that are required specifically for signal transmission; their mutation interferes with fusion, although still allowing efficient F-protein processing and cell surface transport. One residue is located adjacent to the fusion peptide, four line a cavity in the base of the F-trimer head, while the sixth residue is located near this cavity. Hydrophobic interactions in the cavity sustain the fusion process and contacts with H. The cavity is flanked by two different subunits of the F-trimer. Tetrameric H-stalks may be lodged in apposed cavities of two F-trimers. Because these insights are based on a PIV5 homology model, the signal receipt mechanism may be conserved among paramyxoviruses.

UR - http://www.scopus.com/inward/record.url?scp=84866527691&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866527691&partnerID=8YFLogxK

U2 - 10.1074/jbc.M112.373308

DO - 10.1074/jbc.M112.373308

M3 - Article

VL - 287

SP - 33026

EP - 33035

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 39

ER -