Base stacking and molecular polarizability: Effect of a methyl group in the 5-position of pyrimidines

Lawrence Sowers, Barbara Ramsay Shaw, W. David Sedwick

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

Substitution of a methyl group in the 5-position of pyrimidines increases melting temperatures and modifies biological properties of DNA. Increased DNA stability is often attributed to hydrophobic interactions between water and the methyl group. However, we present evidence that the major effect of methyl substitution is to increase the molecular polarizability of the pyrimidine, thereby increasing the base stacking. Experimentally determined base stacking interaction constants for free bases in water are shown to correlate well with calculated molecular polarizability and DNA melting temperatures.

Original languageEnglish (US)
Pages (from-to)790-794
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume148
Issue number2
DOIs
StatePublished - Oct 29 1987
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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